RAJI Cell Line human

85011429, human lymph, Lymphoblastoid

• Szinonimák: GM04671 Cells, P1-Raji Cells
• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: RAJI Cell Line human, 85011429
• biológiai forrás: human lymph
• leírás: Human Black, Burkitt′s lymphoma
• növekedési mód: Suspension
• kariotípus: 2n = 46, diploid
• morfológia: Lymphoblastoid
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Burkitt′s lymphoma

Sejtvonal leírása

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

Alkalmazás

Resistance and virus studies: vesicular stomatitis virus (VSV).
RAJI Cell Line human has been used to investigate the transport of V. parahaemolyticus across the Payer′s patch of the intestinal epithelium and it has been co-cultured with Caco-2 (human colorectal adenocarcinoma cell-line) monolayers to study bacterial translocation.

DNS profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19

Táptalaj

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Egyéb megjegyzések

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