NS0-Turbodoma

6020202, mouse blood, lymphoblast

• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: NS0-Turbodoma, 06020202
• biológiai forrás: mouse blood
• növekedési mód: Not specified
• kariotípus: Not specified
• morfológia: lymphoblast
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse myeloma, serum-free, without cholesterol

Sejtvonal leírása

NS0 cell line (Sigma Catalogue no. 85110503) adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma (Cell Culture Technologies). Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.

Táptalaj

Turbodoma medium (Cell Culture Technologies) + 2mM L-Glutamine (G7513). When freezing cells down use TurboDoma^™ medium (50:50 conditioned medium:fresh medium) + 2mM L-Glutamine (G7513) + 10% DMSO.

Szubkultúra rutin

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

Egyéb megjegyzések

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Jogi információk

TurboDoma is a trademark of Cell Culture Technologies

Jogi nyilatkozat

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.