C8482

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• Szinonimák: Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
• MDL-szám: MFCD03458215
• UNSPSC kód: 41106305
• NACRES: NA.32

Tulajdonságok

• rekombináns: expressed in E. coli
• Minőségi szint: 200
• Teszt: >90% (SDS-PAGE)
• Forma: buffered aqueous glycerol solution
• specifikus aktivitás: >1000 units/mg protein
• molekulatömeg: 43.5 kDa
• UniProt elérési szám: P17612
• kiszállítva: dry ice
• tárolási hőmérséklet: −70°C
• Géninformáció: human ... PRKACA(5566)

Leírás

Biokémiai/fiziológiai hatások

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Fizikai forma

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Analízis megjegyzés

One unit will transfer 1 nanomole of phosphate from ³²P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
K_m (ATP) = 25 μM at 30 °C, pH 7.4. K_m (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable