539576

PKA, Catalytic Subunit, Bovine Heart

• Szinonimák: cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• biológiai forrás: bovine heart
• Minőségi szint: 100
• Teszt: ≥90% (SDS-PAGE)
• form: liquid
• specifikus aktivitás: ≥20,000 units/μg protein
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; avoid repeated freeze/thaw cycles
• technika/technikák: activity assay: suitable
• alkalmasság: suitable for molecular biology
• kiszállítva: wet ice
• tárolási hőmérséklet: −70°C

Leírás

Általános leírás

Research area: Cell Signaling

Native PKA catalytic subunit purified from bovine heart. Protein Kinase A (PKA) is a serine protein kinase that targets serine and threonine residues.

Biokémiai/fiziológiai hatások

Protein kinases are crucial for integrating signaling networks within eukaryotic cells. It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

Kiszerelés

Please refer to vial label for lot-specific concentration.

Figyelmeztetés

Toxicity: Standard Handling (A)

Egység definíció

One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.

Fizikai forma

In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

Feloldás

Following initial thaw, aliquot and freeze (-70°C).

Egyéb megjegyzések

Corbin, J.D., et al. 1988. Methods Enzymol159, 74.
Rannels, S.R., et al 1983. Methods Enzymol99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem.66, 253.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable