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A1231

Sigma-Aldrich

Anti-Aurora-A Kinase antibody, Mouse monoclonal

clone 35C1, purified from hybridoma cell culture

Szinonimák:

Monoclonal Anti-Aurora-A Kinase, Monoclonal Anti-Aurora-A Kinase antibody produced in mouse, Anti-ARK-1, Anti-AURORA2, Anti-Aurora-related kinase 1, Anti-BTAK, Anti-STK15, Anti-STK6, Anti-Serine/threonine protein kinase 15

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About This Item

MDL-szám:
MFCD06411780
UNSPSC kód:
12352203
eCl@ss:
32160410
NACRES:
NA.44

biológiai forrás

mouse

Minőségi szint

200

rekombináns

expressed in mouse cell line

konjugátum

unconjugated

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

35C1, monoclonal

form

PBS solution

molekulatömeg

antigen ~46 kDa

faj reaktivitás

human, mouse

koncentráció

~2 mg/mL

technika/technikák

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract

izotípus

IgG2b

UniProt elérési szám

O14965

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... AURKA(6790) , AURKA(6790)
mouse ... Aurka(20878) , Aurka(20878)

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Általános leírás

Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome.

Egyediség

Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.

Immunogen

recombinant human Aurora-A kinase.

Alkalmazás

Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry.
Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.

Biokémiai/fiziológiai hatások

Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans .
Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr15 by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser353. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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kapcsolódó termék

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Árazás

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Qian He et al.
Molecular biology of the cell, 25(11), 1715-1729 (2014-04-04)
We show here that human embryonic stem (ES) and induced pluripotent stem cell-derived neuroprogenitors (NPs) develop primary cilia. Ciliogenesis depends on the sphingolipid ceramide and its interaction with atypical PKC (aPKC), both of which distribute to the primary cilium and
Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2M transition
Dutertre S, et al.
Journal of Cell Science, 117(12), 2523-2531 (2004)
Edward J Morris et al.
Nature communications, 8, 15289-15289 (2017-05-06)
Cancer cells frequently have amplified centrosomes that must be clustered together to form a bipolar mitotic spindle, and targeting centrosome clustering is considered a promising therapeutic strategy. A high-content chemical screen for inhibitors of centrosome clustering identified Stattic, a Stat3
Shulan Sun et al.
Cancer communications (London, England), 41(9), 851-866 (2021-07-13)
Increasing studies have reported that oncogenes regulate components of the immune system, suggesting that this is a mechanism for tumorigenesis. Aurora kinase A (AURKA), a serine/threonine kinase, is involved in cell mitosis and is essential for tumor cell proliferation, metastasis
Sisi Li et al.
Heliyon, 9(7), e17386-e17386 (2023-07-07)
The contribution of oncogenes to tumor-associated RNA splicing and the relevant molecular mechanisms therein require further elaboration. Here, we show that oncogenic Aurora kinase A (AURKA) promotes breast cancer-related RNA aberrant splicing in a context-dependent manner. AURKA regulated pan-breast cancer-associated
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