Összes fotó(2)
Fontos dokumentumok
A5102
Anti-Aurora B antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Szinonimák:
Anti-AIM-1, Anti-AIR-2 Kinase, Anti-AIRK-2
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
konjugátum
unconjugated
antitest forma
IgG fraction of antiserum
antitest terméktípus
primary antibodies
klón
polyclonal
form
buffered aqueous solution
molekulatömeg
antigen 41 kDa
faj reaktivitás
rat, mouse, human
kiszerelés
antibody small pack of 25 μL
technika/technikák
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:50 using HeLa cells
microarray: suitable
western blot: 1:1,000 using nuclei-enriched fraction of mouse NIH-3T3 cells
western blot: 1:1,000 using using PC-12 rat phaeochromocytoma cells
UniProt elérési szám
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... AURKB(9212)
mouse ... Aurkb(20877)
rat ... Aurkb(114592)
Általános leírás
Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae IpI1 kinase are highly homologous to human Aurora B.
Egyediség
Anti-Aurora B recognizes human, mouse, and rat Aurora B/AIR-2 Kinase enzymes. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.
Immunogen
synthetic peptide corresponding to amino acid residues 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.
Alkalmazás
Anti-Aurora B antibody has been used in:
- in immunoblotting
- in immunoprecipitation
- in immunofluorescence
- in immunofluorescence staining
- in western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit polyclonal anti-Aurora B antibody is used to tag Aurora B/AIR-2 Kinase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Aurora B/AIR-2 Kinase in chromosome segregation, cytokinesis, and cancer development.
Biokémiai/fiziológiai hatások
Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G2M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines.
Cél megnevezése
Auroa B is a member of a family of Auora kinases that function for proper chromosomal segregation during mitosis. Auora B localizes specifically to the kinetochores of microtubules.
Fizikai forma
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
nwg
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Combining neuropeptide Y and mesenchymal stem cells reverses remodeling after myocardial infarction
Wang Y, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 298(1), H275-H286 (2009)
Mechanism of Aurora B activation by INCENP and inhibition by hesperadin
Sessa F, et al.
Molecular Cell, 18(3), 379-391 (2005)
Aurora B regulates MCAK at the mitotic centromere
Andrews P D, et al.
Developmental Cell, 6(2), 253-268 (2004)
Shi-Zheng Wu et al.
Cell biochemistry and function, 35(2), 113-123 (2017-02-25)
It has been reported that CXCR4-overexpressing mesenchymal stem cells (MSCCX4 ) can repair heart tissue post myocardial infarction. This study aims to investigate the MSCCX4-derived paracrine cardio-protective signaling in the presence of myocardial infarction. Mesenchymal stem cells (MSCs) were divided
Yu-Chu Wang et al.
Experimental & molecular medicine, 50(6), 70-70 (2018-06-10)
Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis
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