PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• Szinonimák: Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• UNSPSC kód: 41105323
• NACRES: NA.21

Tulajdonságok

• Terméknév: Tris Buffered Saline, with 0.05% Tween^® 20, pH 8.0, powder, pHast Pack^™
• termékcsalád: pHast Pack^™
• Forma: powder
• tárolási körülmény: (Tightly closed. Dry.)
• szennyeződések: ≤5 ppm Heavy Metals (lead)
• szín: white
• pH: 7.8—8.2
• oldhatóság: soluble, clear to hazy, colorless
• kation nyomok: Fe: ≤10 ppm
• idegen aktivitás: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• tárolási hőmérséklet: room temp

Leírás

Általános leírás

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

Alkalmazás

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

Tulajdonságok és előnyök

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

Kiszerelés

Foil pouches

Feloldás

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Tárolás és stabilitás

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Egyéb megjegyzések

For additional information on our range of Biochemicals, please complete this form.

Jogi információk

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable