Összes fotó(1)
Fontos dokumentumok
10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Szinonimák:
lambda dna
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
Minőségi szint
Forma
solution
molekulatömeg
32,300 kDa
kiszerelés
pkg of 1 mL
gyártó/kereskedő neve
Roche
tárolási körülmény
avoid repeated freeze/thaw cycles
koncentráció
250 μg/mL
szennyeződések
RNA, none detected
nuclease, none detected
szín
colorless
oldhatóság
water: miscible
kiszállítva
dry ice
tárolási hőmérséklet
−20°C (−15°C to −25°C)
Általános leírás
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Alkalmazás
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Minőség
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Szekvencia
Chain Length 48,502 bp
Fizikai forma
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Tárolás és stabilitás
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
nwg
Lobbanási pont (F)
No data available
Lobbanási pont (C)
No data available
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