OP03
Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
liquid, clone PAb421, Calbiochem®
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.43
klón:
PAb421, monoclonal
application:
faj reaktivitás:
rabbit, monkey, human, mouse, rat
citations:
56
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
PAb421, monoclonal
Forma
liquid
tartalmaz
≤0.1% sodium azide as preservative
faj reaktivitás
rabbit, monkey, human, mouse, rat
gyártó/kereskedő neve
Calbiochem®
tárolási körülmény
do not freeze
izotípus
IgG2a
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... TP53(7157)
Általános leírás
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.
Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).
Immunogén
Epitope: within amino acids 376-378 of human p53
Mouse
partially purified mouse p53
Alkalmazás
Frozen Sections (10 µg/ml, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Gel Shift (see comments)
Immunoblotting (10 µg/ml, see application references)
Immunofluorescence (1-20 µg/ml, see application references)
Flow Cytometry (1-20 µg/ml)
Immunoprecipitation (1 μg per sample, see application references)
Kiszerelés
Please refer to vial label for lot-specific concentration.
Figyelmeztetés
Toxicity: Standard Handling (A)
Fizikai forma
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analízis megjegyzés
Negative Control
SK-OV-3 cells or normal skin
SK-OV-3 cells or normal skin
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
Egyéb megjegyzések
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.
Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
Jogi információk
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
10 - Combustible liquids
WGK
nwg
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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