MABS61
Anti-PARG Antibody, clone D8B10
clone D8B10, from mouse
Szinonimák:
poly (ADP-ribose) glycohydrolase, poly(ADP-ribose) glycohydrolase 60 kDa isoform
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
D8B10, monoclonal
faj reaktivitás
human
technika/technikák
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG2aκ
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... PARG(8505)
Általános leírás
Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme possessing both endo- and exoglycosidase activity against poly (ADP-ribose) (PARP), rapidly degrading PARP to release large quantities of free ADP-ribose. PARG is involved in several cellular processes including; apoptosis, DNA repair, cell cycle progression, cell survival and cellular differentiation. During apoptosis, PARG is cleaved by caspase-3 suggesting that PARG activity is regulated during this process. Although encoded by one gene, PARG is present in different cellular localizations as different isoforms: Isoform 1 (111 kDa) is present in the nucleus, Isoform 2 (102 kDA) is in the cytoplasm and Isoform 3 (99 kDa) is mitochondrial. Studies have indicated a critical role for PARG isoform 1 in the quality of sperm chromatin, and subsequent embryonic survival.
Immunogén
Epitope: Unknown
Histidine-tagged recombinant protein corresponding to human PARG.
Alkalmazás
Anti-PARG Antibody, clone D8B10 is an antibody against PARG for use in WB & IP.
Research Category
Signaling
Epigenetics & Nuclear Function
Signaling
Epigenetics & Nuclear Function
Research Sub Category
Hormones & Receptors
Chromatin Biology
Hormones & Receptors
Chromatin Biology
Minőség
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PARG on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PARG on 10 µg of Jurkat cell lysate.
Cél megnevezése
~ 102 kDa observed. Other isoforms may be observed in some lysates at 111 and 99 kDa
Fizikai forma
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Analízis megjegyzés
Control
Jurkat cell lysate
Jurkat cell lysate
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Kaede Tsuda et al.
Cancer reports (Hoboken, N.J.), 6(2), e1709-e1709 (2022-09-03)
Poly(ADP-ribose) glycohydrolase (PARG) is a key enzyme in poly(ADP-ribose) (PAR) metabolism and a potential anticancer target. Many drug candidates have been developed to inhibit its enzymatic activity. Additionally, PDD00017273 is an effective and selective inhibitor of PARG at the first
Jean-Christophe Amé et al.
Methods in molecular biology (Clifton, N.J.), 1608, 395-413 (2017-07-12)
The purification of Poly(ADP-ribose) glycohydrolase (PARG) from overexpressing bacteria Escherichia coli is described here to a fast and reproducible one chromatographic step protocol. After cell lysis, GST-PARG-fusion proteins from the crude extract are affinity purified by a Glutathione 4B Sepharose
Yajie Zhang et al.
Nature methods, 10(10), 981-984 (2013-08-21)
Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear
Xiaoxing Feng et al.
Molecular cancer, 11, 48-48 (2012-07-31)
Cell death induced by poly(ADP-ribose) (PAR) and mediated by apoptosis-inducing factor (AIF) is well-characterized in models of ischemic tissue injury, but their roles in cancer cell death after chemotherapy are less understood. Here we investigated the roles of PAR and
Yukihide Watanabe et al.
The Journal of biological chemistry, 291(24), 12706-12723 (2016-04-30)
We previously established a mechanism of negative regulation of transforming growth factor β signaling mediated by the nuclear ADP-ribosylating enzyme poly-(ADP-ribose) polymerase 1 (PARP1) and the deribosylating enzyme poly-(ADP-ribose) glycohydrolase (PARG), which dynamically regulate ADP-ribosylation of Smad3 and Smad4, two
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