MABE329

Anti-c-Fos Antibody

mouse monoclonal, 2G9C3

• Szinonimák: Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: 2G9C3, monoclonal
• application: ICC; WB
• faj reaktivitás: human
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• citations: 2

Tulajdonságok

• Terméknév: Anti-c-Fos Antibody, clone 2G9C3, clone 2G9C3, from mouse
• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: purified antibody
• antitest terméktípus: primary antibodies
• klón: 2G9C3, monoclonal
• faj reaktivitás: human
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• izotípus: IgG1κ
• NCBI elérési szám: NP_005243
• UniProt elérési szám: P01100
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... FOS(2353)

Leírás

Általános leírás

c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Egyediség

This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins.

Immunogén

Linear peptide corresponding to human c-Fos.

Alkalmazás

Anti-c-Fos Antibody, clone 2G9C3 is a Mouse Monoclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB, ICC.

Immunocytochemistry Analysis: A representative lot detected c-Fos in schwann cells. (Lafarga, M., et al. (2002). Molecular Biology of the Cell. 13:2771–2782.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Minőség

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos on 10 µg of HeLa cell lysate.

Cél megnevezése

~55/62 kDa observed. This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. The calculated molecular wieght is 46 kDa; however, c-Fos has been observed at ~55 kDa in western blots. (Assim, M., et al. (2010). Journal of Biological Chemistry. 285(26):20343–20357.)

Fizikai forma

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ from cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
HeLa cell lysate

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable