MAB3560
Anti-8-Oxoguanine Antibody, clone 483.15
ascites fluid, clone 483.15, Chemicon®
Szinonimák:
8-oxoG
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
483.15, monoclonal
application:
ChIP
ELISA
ICC
ELISA
ICC
faj reaktivitás:
monkey, human, primate
technika/technikák:
ChIP: suitable
ELISA: suitable
immunocytochemistry: suitable
ELISA: suitable
immunocytochemistry: suitable
citations:
56
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
ascites fluid
antitest terméktípus
primary antibodies
klón
483.15, monoclonal
faj reaktivitás
monkey, human, primate
faj reaktivitás (homológia által előrejelzett)
mouse, rat
gyártó/kereskedő neve
Chemicon®
technika/technikák
ChIP: suitable
ELISA: suitable
immunocytochemistry: suitable
izotípus
IgM
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... OGG1(4968)
Általános leírás
8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer′s and Parkinson′s.
Egyediség
8-oxoguanine. By competitive ELISA the monoclonal showed no reactivity with dGMP, dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range.
Immunogén
8-oxoguanine adsorbed on to alumina
Alkalmazás
Anti-8-Oxoguanine Antibody, clone 483.15 detects level of 8-Oxoguanine & has been published & validated for use in ELISA & IC.
Immunocytochemistry:
On HeLa and Cos7 cells fixed with paraformaldehyde. 8-oxoguanine has been localized to the nucleus in nutrient-deprived cells.
ELISA:
Cell grown in slides were extracted twice for 30 sec with cold 560nM NaCl;0.1% (v/v) Triton X-100;0.02% (w/v) SDS;10mM phosphate buffer pH 7.4 and fixed with freshly prepared 4% PFA for 5-20 minutes at room temperature. {Conlon, KA et al (2000) J. Histotechnology 23(1):37-44}. Typical staining shows that nuclei from extracted cells have define periphery and areas of condensed chromatin. Clone 483.15 staining showed specific but faint nuclear fluorescence staining in cells incubated in supplemented DMEM, but in cells incubated in nutrient-free defined solt solution (NFDSS) {1.8mM calcium chloride, 110mM NaCl, 44mM sodium biocarbonate, pH 7.5} showed strong nuclear fluorescence staining that appeared punctate and gernerally distributed. Fluorescence staining disappears to background levels in cells incubated in nutrient medias even after initial NFDSS treatments {Conlon et al}.
A previous lot of this antibody was used in an ELISA assay.
Optimal working dilutions must be determined by end user.
On HeLa and Cos7 cells fixed with paraformaldehyde. 8-oxoguanine has been localized to the nucleus in nutrient-deprived cells.
ELISA:
Cell grown in slides were extracted twice for 30 sec with cold 560nM NaCl;0.1% (v/v) Triton X-100;0.02% (w/v) SDS;10mM phosphate buffer pH 7.4 and fixed with freshly prepared 4% PFA for 5-20 minutes at room temperature. {Conlon, KA et al (2000) J. Histotechnology 23(1):37-44}. Typical staining shows that nuclei from extracted cells have define periphery and areas of condensed chromatin. Clone 483.15 staining showed specific but faint nuclear fluorescence staining in cells incubated in supplemented DMEM, but in cells incubated in nutrient-free defined solt solution (NFDSS) {1.8mM calcium chloride, 110mM NaCl, 44mM sodium biocarbonate, pH 7.5} showed strong nuclear fluorescence staining that appeared punctate and gernerally distributed. Fluorescence staining disappears to background levels in cells incubated in nutrient medias even after initial NFDSS treatments {Conlon et al}.
A previous lot of this antibody was used in an ELISA assay.
Optimal working dilutions must be determined by end user.
Minőség
Routinely evaluated by immunocytochemistry on NIH/3T3 cells..
Immunocytochemistry:
Confocal fluorescent analysis of NIH/3T3 cells using anti-8-oxoguanine mouse monoclonal antibody.
Immunocytochemistry:
Confocal fluorescent analysis of NIH/3T3 cells using anti-8-oxoguanine mouse monoclonal antibody.
Fizikai forma
Unpurified mouse monoclonal IgM liquid in buffer containing no preservative.
Tárolás és stabilitás
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance.
Analízis megjegyzés
Control
Nutrient starved HeLa or Cos7 cells
Nutrient starved HeLa or Cos7 cells
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
10 - Combustible liquids
WGK
nwg
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Robert Lowe et al.
Genome biology, 16, 194-194 (2015-09-19)
Cellular senescence is a stable arrest of proliferation and is considered a key component of processes associated with carcinogenesis and other ageing-related phenotypes. Here, we perform methylome analysis of actively dividing and deeply senescent normal human epithelial cells. We identify
Vascular effects of a low-carbohydrate high-protein diet.
Foo, SY; Heller, ER; Wykrzykowska, J; Sullivan, CJ; Manning-Tobin, JJ; Moore et al.
Proceedings of the National Academy of Sciences of the USA null
Ilir Mehmeti et al.
Biochimica et biophysica acta, 1813(10), 1827-1835 (2011-07-26)
Pro-inflammatory cytokine-mediated beta cell apoptosis is activated through multiple signaling pathways involving mitochondria and endoplasmic reticulum. Activation of organelle-specific caspases has been implicated in the progression and execution of cell death. This study was therefore performed to elucidate the effects
Sameera Nallanthighal et al.
Nanotoxicology, 11(8), 996-1011 (2017-10-20)
Due to extensive use in consumer goods, it is important to understand the genotoxicity of silver nanoparticles (AgNPs) and identify susceptible populations. 8-Oxoguanine DNA glycosylase 1 (OGG1) excises 8-oxo-7,8-dihydro-2-deoxyguanine (8-oxoG), a pro-mutagenic lesion induced by oxidative stress. To understand whether
Sameera Nallanthighal et al.
NanoImpact, 5, 92-100 (2017-09-26)
Incorporation of silver nanoparticles (AgNPs) in toothpaste, food containers, dietary supplements and other consumer products can result in oral exposure to AgNPs and/or silver ions (Ag+) released from the surface of AgNPs. To examine whether ingestion of AgNPs or Ag+
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