MAB3199
Anti-E-Cadherin Antibody, clone 67A4
clone 67A4, Chemicon®, from mouse
Szinonimák:
CD324
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
67A4, monoclonal
application:
FACS
WB
WB
faj reaktivitás:
human
technika/technikák:
flow cytometry: suitable
western blot: suitable
western blot: suitable
citations:
7
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
67A4, monoclonal
faj reaktivitás
human
gyártó/kereskedő neve
Chemicon®
technika/technikák
flow cytometry: suitable
western blot: suitable
izotípus
IgG1
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... CDH1(999)
Egyediség
Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E-cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.
Antigen is expressed in non-neural epithelial tissues.
Antigen is expressed in non-neural epithelial tissues.
Immunogén
T-47D breast carcinoma line
Alkalmazás
Anti-E-Cadherin Antibody, clone 67A4 detects level of E-Cadherin & has been published & validated for use in FC & WB.
Immunoblotting: 123kD in membrane fractions; MCF-7 membranes positive control. Confluent cells were washed three times with ice-cold PBS and lysed in 50mM HEPES pH 7.5, 150mM NaCl, 1mM EDTA, 10% glycerine, 1% triton X-100, 20mM Na(4)P(2)O(7), 2mM Na-vanadate, 100mM NaF, 2mM PMSF, 20μg/mL leupeptin, 1mM pNPP(para-nitrophenylphosphate), 20μg/mL aprotinin, and 200μM NH(4)-molybdate. Crude extracts were clarified by centrifugation (10,000 x g 10 min), and supernatants were taken for immunoprecipitation. Protein concentration of cleared lysates were determined by BCA protein assay. {Buhring, et al Luekemia 10:106-116, 1996}.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Original paper, Buhring, H et al Leukemia 10:106-116, 1996.
Optimal working dilutions must be determined by end user.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Original paper, Buhring, H et al Leukemia 10:106-116, 1996.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Kapcsolódás
Replaces: 04-1103
Fizikai forma
Format: Purified
Protein A purified immunoglobulin. Liquid in 0.02M PBS with NaCl, pH=7.6, 0.1% Sodium azide.
Tárolás és stabilitás
Maintain 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
J C Evans et al.
Gene therapy, 22(10), 802-810 (2015-05-26)
Prostate cancer is the most common cancer in men of the western world. To date, no effective treatment exists for metastatic prostate cancer and consequently, there is an urgent need to develop new and improved therapeutics. In recent years, the
Li Li et al.
Biophysical journal, 98(11), 2442-2451 (2010-06-02)
Clonality is, at present, the only means by which the self-renewal potential of a given stem cell can be determined. To assess the clonality of human embryonic stem cells (hESC), a protocol involving seeding wells at low cell densities is
Claudia M García-Cuellar et al.
International journal of molecular sciences, 22(23) (2021-12-11)
Air pollution presents a major environmental problem, inducing harmful effects on human health. Particulate matter of 10 μm or less in diameter (PM10) is considered an important risk factor in lung carcinogenesis. Epithelial-mesenchymal transition (EMT) is a regulatory program capable
Androgen receptor-independent function of FoxA1 in prostate cancer metastasis.
Jin, HJ; Zhao, JC; Ogden, I; Bergan, RC; Yu, J
Cancer Research null
Zhongyu Liu et al.
Biomedical optics express, 10(10), 5414-5430 (2019-10-28)
We have developed a human bronchial epithelial (HBE) cell and endothelial cell co-cultured microfluidic model to mimic the in vivo human airway. This airway-on-a-chip was designed with a central epithelial channel and two flanking endothelial channels, with a three-dimensional monolayers
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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