MAB1949
Anti-Laminin-5 Antibody, clone P3E4
clone P3E4, Chemicon®, from mouse
Szinonimák:
Epiligrin
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
P3E4, monoclonal
faj reaktivitás
human
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG1
alkalmasság
not suitable for activity/function inhibition
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... LAMC2(3918)
Általános leírás
Epiligrin, the major component of human keratinocyte extracellular matrix, serves as the preferred integrin ligand for alpha 3 beta 1 in plasma membranes and focal adhesions, and colocalizes with alpha 6 beta 4 in hemidesmosomes. {Domloge-Hultsch, et al (1992) J Clin Invest 90(4):1628-1633}. Major glycoprotein of epidermal basement membrane, consisting of three disulphide bonded subunits of 170, 145 and 135 kD. Epiligrin is the major ligand for alpha3/beta1 integrin, is particularly prominent in the lamina lucida of the skin and is absent in patients with lethal junctional epidermolysis bullosa. Today, epiligrin is identical to laminin 5, also called BM600, nicein, and kalinin. Laminin 5 (epiligrin; Carter et al, 1991 Cell 65(4):599-610) as the primary ligand in epithelial BMs that mediates both cell anchorage (adhesion without migration) via integrin a6b4 in homeostatic tissue and cell migration via integrin a3b1 in wound repair.
The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.
The laminin-5 isoform (nicein, epiligrin, and kalinin) is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa, and other epithelial glands (Kallunki et al., 1992; Stahl et al., 1997). Laminin-5 is a heterotrimer consisting of alpha3, beta3, and gamma2 subunits that associate via large helical regions to produce a cruciform-shaped molecule (Rousselle et al., 1991 J. Cell Biol. 114: 567-576; Baker et al., 1996 J. Cell Sci. 109: 2509-2520). Laminin-5 is synthesized initially as a 460-kD molecule that undergoes specific processing to a smaller form after being secreted into the extracellular matrix (Marinkovich et al., 1992 J. Biol. Chem. 267: 17900-17906; Vailly et al., 1994 Eur. J. Biochem. 219: 209-218; Matsui et al., 1995 J. Biol. Chem. 270: 23496-23503). The size reduction is a result of processing the 3 and 2 subunits from 190-200 to 160 kD and from 155 to 105 kD, respectively (Marinkovich et al., 1992; Vailly et al., 1994; Matsui et al., 1995). {Goldfinger, LE (1998) J Cell Biol 141(1):255-265}.
Egyediség
Human epiligrin (laminin 5)
Immunogen
Human keratinocytes
Alkalmazás
Immunohistochemistry: for use on acetone fixed tissue
Immunocytochemistry
Immunoblotting: (non-reducing)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunoblotting: (non-reducing)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Laminin-5 Antibody, clone P3E4 is validated for use in ELISA, IP, WB, IC, IH for the detection of Laminin-5.
Fizikai forma
Format: Purified
Please see datasheet for lot-specific information.
Tárolás és stabilitás
Store at +2 to 8°C.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Timothy D Culp et al.
Virology, 347(1), 147-159 (2005-12-27)
Human papillomaviruses (HPVs) have previously been shown to adsorb to cultured cells via membrane-associated heparan sulfate (HS) and alpha6 integrin. We demonstrate that cultured keratinocytes uniquely secrete a component into the basal extracellular matrix (ECM) which can function to adsorb
M Barzegar et al.
The British journal of dermatology, 169(6), 1353-1356 (2013-07-23)
Laryngo-onycho-cutaneous (LOC) syndrome is a subtype of autosomal recessive junctional epidermolysis bullosa in which there is prominent skin and mucosal granulation tissue that can lead to delayed wound healing, laryngeal obstruction and blindness. Thus far, all cases are of Punjabi
Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2.
Kondo, S; Tang, Y; Scheef, EA; Sheibani, N; Sorenson, CM
American Journal of Physiology. Cell Physiology null
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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