AB756P
Anti-Collagen Antibody, Type IV
Chemicon®, from rabbit
Szinonimák:
Anti-ATS2, Anti-CA44
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ELISA
IF
IHC
IF
IHC
faj reaktivitás:
mouse
technika/technikák:
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
citations:
148
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
mouse
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
alkalmasság
not suitable for Western blot
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Általános leírás
Collagen Type IV extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III and mouse Fibronectin and Laminin.
Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.
Egyediség
Antibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III, mouse Fibronectin, and mouse laminin.
Mouse Collagen, Type IV: 100 (%’s at a 1:5000 RIA dilution) Mouse Collagen, Types I, II, III: <0.1 Human Collagen, Types IV, V: <0.1 Mouse Fibronectin: <0.1 Mouse Laminin: <0.1 Reactivity with other species has not been determined.
Immunogén
Collagen Type IV extracted and purified from mouse tumor tissues.
Alkalmazás
ELISA:
A previous lot of this antibody was used in ELISA at >1:200 (OD >500).
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescence.
Immunohistochemistry:
1:80 dilution for immunofluorescent staining of fresh frozen mouse skin and liver tissues. Acetone or methyl-carnoy fixed paraffin-embedded tissue (mouse skin and liver) is also reactive.
Not recommended for Western blots.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in ELISA at >1:200 (OD >500).
Immunofluorescence:
A previous lot of this antibody was used in immunofluorescence.
Immunohistochemistry:
1:80 dilution for immunofluorescent staining of fresh frozen mouse skin and liver tissues. Acetone or methyl-carnoy fixed paraffin-embedded tissue (mouse skin and liver) is also reactive.
Not recommended for Western blots.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Collagen Antibody, Type IV is validated for use in ELISA, IF, IH(P) for the detection of Collagen.
Cél megnevezése
139 kDa (unprocessed precursor)
Kapcsolódás
Replaces: AB756
Fizikai forma
Format: Purified
Protein G Purified
The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.
Tárolás és stabilitás
Stable for 1 year at -20ºC from date of receipt.
Analízis megjegyzés
Control
Positive Control: Kidney, muscle, tendon spleen tissue, mouse liver. Negative Control: Neurons/glia.
Positive Control: Kidney, muscle, tendon spleen tissue, mouse liver. Negative Control: Neurons/glia.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Magdalena Leiva et al.
Nature communications, 7, 10222-10222 (2016-01-09)
The life-long maintenance of haematopoietic stem and progenitor cells (HSPCs) critically relies on environmental signals produced by cells that constitute the haematopoietic niche. Here we report a cell-intrinsic mechanism whereby haematopoietic cells limit proliferation within the bone marrow, and show
Long-term retinal PEDF overexpression prevents neovascularization in a murine adult model of retinopathy.
Haurigot, V; Villacampa, P; Ribera, A; Bosch, A; Ramos, D; Ruberte, J; Bosch, F
Testing null
Fabienna Arends et al.
PloS one, 10(2), e0118090-e0118090 (2015-02-18)
The migration of cells within a three-dimensional extracellular matrix (ECM) depends sensitively on the biochemical and biophysical properties of the matrix. An example for a biological ECM is given by reconstituted basal lamina gels purified from the Engelbreth-Holm-Swarm sarcoma of
Ilkka Pietilä et al.
PloS one, 11(1), e0147171-e0147171 (2016-01-23)
The Wnts can be considered as candidates for the Congenital Anomaly of Kidney and Urinary Tract, CAKUT diseases since they take part in the control of kidney organogenesis. Of them Wnt5a is expressed in ureteric bud (UB) and its deficiency
Katherine Rybinski et al.
Oncotarget, 6(28), 25429-25440 (2015-09-04)
Over-expression of endosialin/CD248 (herein referred to as CD248) has been associated with increased tumor microvasculature in various tissue origins which makes it an attractive anti-angiogenic target. In an effort to target CD248, we have generated a human CD248 knock-in mouse
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