AB5585-I

Anti-Recoverin Antibody

from rabbit, purified by affinity chromatography

• Szinonimák: Cancer-associated retinopathy protein, Cancer-associated retinopathy antigen, Protein CAR, Recoverin
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: human, rat
• faj reaktivitás (homológia által előrejelzett): mouse (based on 100% sequence homology)
• technika/technikák: immunohistochemistry: suitable; western blot: suitable
• NCBI elérési szám: NP_002894
• UniProt elérési szám: P35243
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: mouse ... Rcvrn(19674)

Leírás

Általános leírás

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. Recoverin is initially produced as a 200-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. Recoverin is composed of four EF-hand domains (a.a. 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. In photoreceptor cells, recoverin functions as a Ca2+-dependent inhibitor of G-protein-coupled receptor kinase 1 (GRK1). Recoverin plays an important role in mediating calcium-dependent rod and cone light adaptation. Recoverin delays the rod & cone response under dim light when [Ca2+] is high by inhibiting GRK1-catalyzed visual pigment phosphorylation, but has little effect during light adaptation when [Ca2+]i is lowered.

Immunogen

Epitope: EF-hand 4

KLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin.

Alkalmazás

Anti-Recoverin Antibody detects level of Recoverin and has been published and validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human and rat retina tissue sections.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Minőség

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Recoverin in 10 µg of human retina tissue lysate.

Cél megnevezése

~23 kDa observed.

Fizikai forma

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable