AB3565

Anti-PARP (214/215) cleavage site Antibody

Chemicon^®, from rabbit

• Szinonimák: Poly [ADP-ribose] polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, ARTD1
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: polyclonal
• application: ICC; WB
• faj reaktivitás: mouse, bovine, rat, human
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• citations: 16

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: mouse, bovine, rat, human
• gyártó/kereskedő neve: Chemicon^®
• technika/technikák: immunocytochemistry: suitable; western blot: suitable
• NCBI elérési szám: NM_001618.3
• UniProt elérési szám: P09874
• kiszállítva: dry ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: bovine ... Parp1(286764) ; human ... PARP1(142) ; mouse ... Parp1(11545) ; rat ... Parp1(25591)

Leírás

Általános leírás

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

Egyediség

Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.

The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

Immunogén

Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

Alkalmazás

Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.

For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry

Minőség

Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.

Kapcsolódás

Replaces: 04-575; 04-576

Fizikai forma

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

ImmunoAffinity Purified

Tárolás és stabilitás

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Analízis megjegyzés

Control
Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable