524390

Phorbol 12,13-dibutyrate

≥99% (HPLC), solid (crystalline), PKC activator, Calbiochem

• Szinonimák: Phorbol-12,13-dibutyrate, PDBu, PKC Activator II
• Tapasztalati képlet (Hill-képlet): C₂₈H₄₀O₈
• CAS-szám: 37558-16-0
• Molekulatömeg: 504.61
• MDL-szám: MFCD00036783
• UNSPSC kód: 12352211
• NACRES: NA.77

Tulajdonságok

• Terméknév: Phorbol-12,13-dibutyrate, Strong irritant for mouse skin, but only moderately active as a tumor promoter.
• Minőségi szint: 100
• Teszt: ≥99% (HPLC)
• Forma: solid (crystalline)
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze
• szín: white to pale yellow
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C
• SMILES string: O([C@]21[C@H]([C@H]3[C@@]([C@H]4[C@](CC(=C3)CO)(C(=O)C(=C4)C)O)([C@@H]([C@H]2OC(=O)CCC)C)O)C1(C)C)C(=O)CCC
• InChI: 1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1
• Nemzetközi kémiai azonosító kulcs: BQJRUJTZSGYBEZ-YVQNUNKESA-N

Leírás

Általános leírás

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C (PKC). Stimulates the phosphorylation of Na⁺-K⁺ ATPase, thereby inhibiting its activity. Promotes the expression of iNOS in cultured hepatocytes. PDBu can be diluted to 30 µM in aqueous solutions, so it is commonly used in binding studies or in applications requiring high concentrations. It is easier to remove PDBu than PMA from cells in culture.

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C. Stimulates the phosphorylation of Na⁺,K⁺- ATPase, thereby inhibiting its activity. Promotes the expression of inducible NOS in cultured hepatocytes. Commonly used in binding studies or in applications requiring high concentrations of phorbol compounds.

Biokémiai/fiziológiai hatások

Cell permeable: no

Primary Target
Na⁺,K⁺- ATPase

Product does not compete with ATP.

Reversible: no

Kiszerelés

Packaged under inert gas

Figyelmeztetés

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Elkészítési megjegyzés

Further dilute with aqueous buffers just prior to use.

Feloldás

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.

Egyéb megjegyzések

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427.
Lee, E.J., et al. 1994. Brain Res. 665, 127.
Critz, S.D., et al. 1993. J. Neurochem.60, 1175.
Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611.
Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Piktogramok: GHS06,GHS08,GHS05
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H300 + H310 + H330,H314,H317,H334,H351
• Óvintézkedésre vonatkozó mondatok: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Veszélyességi osztályok: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• Tárolási osztály kódja: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable