204888

Complement C5, Human

Complement C5, Human, CAS 80295-53-0. is a native glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds.

• Szinonimák: Complement C5, Human
• CAS-szám: 80295-53-0
• MDL-szám: MFCD00130840
• UNSPSC kód: 12352202
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 200
• Teszt: ≥95% (SDS-PAGE)
• form: liquid
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; avoid repeated freeze/thaw cycles
• szennyeződések: IgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9, trace
• kiszállítva: wet ice
• tárolási hőmérséklet: −70°C

Leírás

Általános leírás

Native, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in the assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at peptide bond 74 resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC).

Native, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120,000 (α) and 75,000 (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at the peptide bond 74, resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form the lytic C5b-9 complement membrane attack complex (MAC).

Biokémiai/fiziológiai hatások

≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis

Kiszerelés

Please refer to vial label for lot-specific concentration.

Figyelmeztetés

Toxicity: Standard Handling (A)

Fizikai forma

In PBS, pH 7.2.

Elkészítési megjegyzés

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Feloldás

Following initial thaw, aliquot and freeze (-70°C).

Egyéb megjegyzések

Janatova, J. 1988. Methods Enzymol. 162, 579.
Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable