06-747
Anti-Neomycin Phosphotransferase II Antibody
Upstate®, from rabbit
Szinonimák:
Anti-Neomycin II, Neomycin II Antibody, Neomycin Phosphotransferase Ab
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
WB
faj reaktivitás:
human, bacteria
technika/technikák:
western blot: suitable
citations:
18
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
human, bacteria
faj reaktivitás (homológia által előrejelzett)
mouse, rat
gyártó/kereskedő neve
Upstate®
technika/technikák
western blot: suitable
izotípus
IgG
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Általános leírás
Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
Egyediség
Recognizes NPT II, Mr ~30 kDa
Immunogén
Full length neomycin phosphotransferase (NPT) II
Alkalmazás
Anti-Neomycin Phosphotransferase II Antibody is an antibody against Neomycin Phosphotransferase II for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western Blot Analysis:
1-2 μg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
1-2 μg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
Minőség
Routinely evaluated by Western Blot on transiently transfected Cos-1 cells.
Western Blot Analysis:
1-2 μg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
Western Blot Analysis:
1-2 μg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
Cél megnevezése
~30 kDa
Fizikai forma
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
Tárolás és stabilitás
Stable for 1 year at 2 - 8°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2 - 8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below 2 - 8°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2 - 8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below 2 - 8°C may cause glycerol containing solutions to become frozen during storage.
Analízis megjegyzés
Control
RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene
RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum.
Wagner, JC; Goldfless, SJ; Ganesan, SM; Lee, MC; Fidock, DA; Niles, JC
Malaria Journal null
Jennifer E Dumaine et al.
eLife, 10 (2021-12-07)
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Trp(250) -hK2 is defective in intracellular trafficking and activates the unfolded protein response.
Eun Ju Choi et al.
Genes to cells : devoted to molecular & cellular mechanisms, 20(6), 512-520 (2015-04-08)
hK2, a member of the kallikrein protease family encoded by KLK2, is expressed exclusively in prostate and is a putative adjunct tumor marker for prostate cancer screening. The T allele of rs198977, a single nucleotide polymorphism in exon 5 of
Jan Kosla et al.
Cell communication and signaling : CCS, 11, 51-51 (2013-08-01)
Although there is extensive evidence for the amoeboid invasiveness of cancer cells in vitro, much less is known about the role of amoeboid invasiveness in metastasis and the importance of Rho/ROCK/MLC signaling in this process. We analyzed the dependence of
Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.
Wagner, JC; Platt, RJ; Goldfless, SJ; Zhang, F; Niles, JC
Nature Methods null
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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