05-485
Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12
clone 9E12, Upstate®, from mouse
Szinonimák:
Acute-phase response factor, DNA-binding protein APRF, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase response factor)
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
9E12, monoclonal
application:
ELISA
IP
WB
IP
WB
faj reaktivitás:
canine, human, mouse
technika/technikák:
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
22
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
9E12, monoclonal
faj reaktivitás
canine, human, mouse
gyártó/kereskedő neve
Upstate®
technika/technikák
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG1κ
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
phosphorylation (pTyr705)
Géninformáció
human ... STAT3(6774)
Általános leírás
STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
Egyediség
This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.
Immunogén
KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3
Alkalmazás
Immunoprecipitation: An independent laboratory has reported 1-10 µg/mL of a previous lot of this antibody immunoprecipitated phospho-STAT3 (Tyr705) from a lysate of 106 vanadate-treated human A431 cells.
ELISA: 0.05 µg/mL of this antibody is recommended by an independent lab for ELISA testing. Test was conducted on a previous lot.
ELISA: 0.05 µg/mL of this antibody is recommended by an independent lab for ELISA testing. Test was conducted on a previous lot.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF. This Stat3 Antibody has been validated in Human, Mouse and Canine samples.
Minőség
Routinely evaluated by immunoblot on lysates from human SK-N-MC cells stimulated with CNTF.
Western Blot Analysis:
0.1-1 µg/mL of this lot detected phospho-STAT3 (Tyr705) in lysates from human SK-NMC cells stimulated with CNTF.
Western Blot Analysis:
0.1-1 µg/mL of this lot detected phospho-STAT3 (Tyr705) in lysates from human SK-NMC cells stimulated with CNTF.
Cél megnevezése
92 kDa
Kapcsolódás
Replaces: 04-1059
Fizikai forma
Format: Purified
Lyophilized purified mouse IgG1ќ from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Thiophilic adsoption and size exclusion chromatography
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analízis megjegyzés
Control
SK-N-MC cells
murine macrophage 4/4 cells, serum-starved for 16 hours then stimulated with Phorbol 12-myristate 13-acetate (PMA) for 10 minutes
SK-N-MC cells
murine macrophage 4/4 cells, serum-starved for 16 hours then stimulated with Phorbol 12-myristate 13-acetate (PMA) for 10 minutes
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Tárolási osztály kódja
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Lei He et al.
American journal of physiology. Gastrointestinal and liver physiology, 316(6), G774-G784 (2019-04-18)
Previous studies suggest that the renin-angiotensin system (RAS) is a pathogenic factor for colitis. The goal of this study was to elucidate the molecular mechanism whereby angiotensin II (ANG II) promotes colonic inflammation. We found that renin was highly induced
Malcolm A Meyn et al.
Science signaling, 2(92), ra64-ra64 (2009-10-15)
Multiple Src family kinases (SFKs) are present in murine embryonic stem (mES) cells. Whereas complete inhibition of SFK activity blocks mES cell differentiation, sole inhibition of the SFK member c-Yes induces differentiation. Thus, individual SFKs may have opposing roles in
Synergistic signaling by corticotropin-releasing hormone and leukemia inhibitory factor bridged by phosphorylated 3',5'-cyclic adenosine monophosphate response element binding protein at the Nur response element (NurRE)-signal transducers and activators of transcription (STAT) element of the proopiomelanocortin promoter.
Mynard, V; Latchoumanin, O; Guignat, L; Devin-Leclerc, J; Bertagna, X; Barre, B; Fagart et al.
Molecular Endocrinology null
Xianping Shi et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 15(5), 1686-1697 (2009-02-26)
Resistance to STI571 is an emerging problem for patients with chronic myelogenous leukemia (CML). Mutation in the kinase domain of Bcr-Abl is the predominant mechanism of the acquired resistance to STI571. In the present study, we investigated the effect of
Ralph A Zirngibl et al.
Molecular and cellular biology, 22(8), 2472-2486 (2002-03-23)
The fps/fes proto-oncogene encodes a cytoplasmic protein tyrosine kinase implicated in growth factor and cytokine receptor signaling and thought to be essential for the survival and terminal differentiation of myeloid progenitors. Fps/Fes-null mice were healthy and fertile, displayed slightly reduced
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