04-1572-I
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
culture supernatant, clone 3E8, from rat
Szinonimák:
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rat
Minőségi szint
antitest forma
culture supernatant
antitest terméktípus
primary antibodies
klón
3E8, monoclonal
faj reaktivitás
mouse
faj reaktivitás (homológia által előrejelzett)
human (immunogen homology)
technika/technikák
ChIP: suitable
ELISA: suitable
western blot: suitable
izotípus
IgG2aκ
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
phosphorylation (pSer5)
Géninformáció
human ... POLR2B(5431)
Általános leírás
RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-5′ CTD residue of RPB1.
Egyediség
This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).
Immunogen
Epitope: Phosphorylated Ser5 at the C-terminus domain (CTD).
Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).
Alkalmazás
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Minőség
Evaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.
Cél megnevezése
~220 kDa observed
Kapcsolódás
Replaces: 04-1572
Fizikai forma
Rat monoclonal IgG2aκ with 0.05% sodium azide.
Unpurified
Tárolás és stabilitás
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
Untreated and lambda phosphotase treated NIH/3T3 cell lysates
Untreated and lambda phosphotase treated NIH/3T3 cell lysates
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
Ghamari, Alireza, et al.
Genes & Development, 27, 767-777 (2013)
Dan Yu et al.
Nucleic acids research, 47(9), 4462-4475 (2019-03-14)
The general transcription factor P-TEFb, a master regulator of RNA polymerase (Pol) II elongation, phosphorylates the C-terminal domain (CTD) of Pol II and negative elongation factors to release Pol II from promoter-proximal pausing. We show here that P-TEFb surprisingly inhibits
Jing Gao et al.
Haematologica (2021-06-25)
Despite significant progress in the treatment of patients with diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL), prognosis of patients with relapsed disease remains poor due to the emergence of drug resistance and subsequent disease progression. Identification of
Rob D Chapman et al.
Science (New York, N.Y.), 318(5857), 1780-1782 (2007-12-15)
RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of
Joshua D Eaton et al.
eLife, 13 (2024-07-08)
RNA polymerase II (RNAPII) transcription initiates bidirectionally at many human protein-coding genes. Sense transcription usually dominates and leads to messenger RNA production, whereas antisense transcription rapidly terminates. The basis for this directionality is not fully understood. Here, we show that
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