04-1572

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

clone 3E8, from rat

• Szinonimák: DNA-directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rat
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: 3E8, monoclonal
• faj reaktivitás: mouse
• faj reaktivitás (homológia által előrejelzett): human (based on 100% sequence homology)
• technika/technikák: ChIP: suitable; ELISA: suitable; western blot: suitable
• izotípus: IgG2aκ
• NCBI elérési szám: NP_000928
• UniProt elérési szám: P24928
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: phosphorylation (pSer5)
• Géninformáció: human ... POLR2B(5431)

Leírás

Általános leírás

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.

Egyediség

This antibody recognizes RNA polymerase II subunit B1 at the CTD when phosphorylated at Ser5.

Immunogen

Epitope: Ser5

Ovalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser5.

Alkalmazás

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins

Use Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 (Rat Monoclonal Antibody) validated in WB, ELISA, ChIP to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5).

Minőség

Evaluated by Western Blot in γ-PPase untreated and treated NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.

Cél megnevezése

~ 220 kDa

Fizikai forma

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable