HPLC Analysis of Proteins on TSKgel^® Butyl-NPR

column

TSKgel Butyl-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (814947)

mobile phase

[A] 0.1 M phosphate buffer, pH 7.0 plus ammonium sulfate, 2.3 M to 0 M in 12 min [B & C] = 0.1 M phosphate buffer, pH 7.0 plus ammonium sulfate, 1.8 M to 0 M in 60 min

flow rate

1 mL/min

detector

UV, 280 nm