HPLC Analysis of Peptides on Discovery^® BIO Wide Pore C5

column

Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)

mobile phase

[A]: 0.1% PFPA (pentafluoropropionic acid) in water:acetonitrile (81:19); [B]: 0.1% PFPA in water:acetonitrile (62:38)

gradient

0-100% B in 19 min

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 215 nm

injection

10 μL ~0.25 μg each peptide

sample

Cat. No. P2693