HPLC Analysis of Carbamate and Urea Pesticides on SUPELCOSIL^™ LC-8 after SPME using 60 μm PDMS/DVB Fiber

sample/matrix

3 mL water containing 8ng/mL of each analyte in 10% NaCl

SPME fiber

PDMS/DVB, 60 μm (57317)

extraction

immersion, 40 min, rapid stirring

desorption process

static, 5 min in acetonitrile:water (65:35); dynamic, valve open during run

column

SUPELCOSIL LC-8, 15 cm × 4.6 mm I.D., 5 μm particles (58220-U)

mobile phase

[A] acetonitrile; [B] water

gradient

18:82 to 65:35 in 9 min, hold 3 min

flow rate

2.0 mL/min

column temp.

35 °C

detector

UV, 240 nm