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GC Analysis of PCBs in Transformer Oil on SPB®-5 after SPE using Supelclean LC-Florisil® and LC-Si

GC Analysis of PCBs in Transformer Oil on SPB®-5 after SPE using Supelclean™ LC-Florisil® and LC-Si suitable for GC, application for SPE

Materiály

analytical column

Č. produktu
Popis
Stanovení ceny

SPB®-5 Capillary GC Column

L × I.D. 30 m × 0.32 mm, df 0.25 μm

SPE tube or plate

Č. produktu
Popis
Stanovení ceny

Supelclean LC-Si SPE Tube

bed wt. 1 g, volume 6 mL, pkg of 30 ea

Supelclean LC-Florisil® SPE Tube

bed wt. 1 g, volume 6 mL, pkg of 30 ea

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

used transormer oil containing 50 ppb Aroclor 1254.

SPE tube/cartridge

Supelclean LC-Florisil, 1.0 g/6 mL (57057)

SPE tube/cartridge

Supelclean LC-Si, 1.0 g/6 mL (57051)

condition

0.5 mL isooctane (Florisil tube)

sample addition

0.2 g transformer oil or 0.1 g waste oil

washing

5 x 2 mL isooctane

column

SPB-5, 30 m × 0.32 mm I.D., 0.25 μm film (24048)

oven

40 °C (4 min) to 300 °C at 10 °C/min, hold 5 min

detector

ECD

carrier gas

nitrogen

injection

1 μL, splitless (30 sec delay), then split (50:1)

Popis

Application

Connect Supelclean LC-Si SPE tube (1 g/6 mL) in series to male luer outlet of Supelclean LC-Florisil SPE tube (1 g/6 mL) using an SPE Tube Adapter (57020-U). Apply up to 0.2 g transformer oil or 0.1 g waste oil to upper frit of Florisil SPE tube. Pass 5 x 2 mL isooctane through both Florisil and LC-Si SPE tubes. Oil interferences will be retained on SPE tubes whereas PCBs will elute with isooctane fractions. Collect and combine isooctane fractions using 10 mL volumetric flask. Analyze isooctane extract using GC-ECD.

Legal Information

Florisil is a registered trademark of U.S. Silica Company
SPB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supelclean is a trademark of Sigma-Aldrich Co. LLC