Přejít k obsahu
Merck
  • Carvacrol and rosemary essential oil manifest cytotoxic, DNA-protective and pro-apoptotic effect having no effect on DNA repair.

Carvacrol and rosemary essential oil manifest cytotoxic, DNA-protective and pro-apoptotic effect having no effect on DNA repair.

Neoplasma (2014-10-25)
M Melusova, D Slamenova, K Kozics, S Jantova, E Horvathova
ANOTACE

For several thousand years natural products were successfully used to treat a variety of diseases and to maintain health in humans, but until now it is not fully known what causes these medicinal effects. In our study we assessed the cytotoxic, DNA-protective and pro-apoptotic effect of two frequently occurring natural compounds, carvacrol and rosemary essential oil, on human hepatoma HepG2 cells. In addition we examined the in vitro incision repair activity of liver cell extracts prepared from hepatocytes isolated from Sprague-Dawley (SD) rats fed with water containing carvacrol or rosemary oil. Using conventional and modified single cell gel electrophoresis we proved that incubation of HepG2 cells with selected concentrations of carvacrol and rosemary oil significantly protected cellular DNA against two dangerous oxidative agents, hydrogen peroxide (H(2)O(2)) and 2,3-dimethoxy-1,4-naphthoquinone (DMNQ). It is interesting that despite this DNA protection, the addition of both volatiles to the drinking water of SD rats had no effect on incision repair capacity of hepatocyte extracts. In this paper we also showed that carvacrol and rosemary oil can trigger apoptotic cell death pathways in HepG2 cells, which is probably connected with their cytotoxicity.

MATERIÁLY
Číslo produktu
Značka
Popis produktu

Supelco
Benzo[a]pyrene solution, certified reference material, TraceCERT®, 1000 μg/mL in acetone
Supelco
Benzo[a]pyrene solution, certified reference material, TraceCERT®, 200 μg/mL in methylene chloride
Sigma-Aldrich
p-Cymene, ≥97%, FG
Sigma-Aldrich
Trypan Blue, ≥80% (HPLC), Dye content 60 %
Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
Sigma-Aldrich
(±)-Camphor, meets analytical specification of Ph. Eur., BP, racemic, ≥95% (GC)
Supelco
Hydrogen peroxide solution, ≥30%, for trace analysis
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
Supelco
1-Methyl-2-pyrrolidinone, analytical standard
Sigma-Aldrich
Ethidium bromide solution, for fluorescence, ~1% in H2O
Sigma-Aldrich
(±)-Camphor, purum, synthetic, ≥95.0% (GC)
Supelco
(−)-Camphor, analytical standard
Sigma-Aldrich
p-Cymene, 99%
Sigma-Aldrich
Camphor, 96%
Sigma-Aldrich
(±)-Camphor, ≥95.5%
Sigma-Aldrich
Trypan Blue, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O
Sigma-Aldrich
Ethidium bromide solution, BioReagent, for molecular biology, 500 μg/mL in H2O
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
USP
Camphor, United States Pharmacopeia (USP) Reference Standard
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
Supelco
4-tert-Octylphenol monoethoxylate solution, 10 μg/mL in acetone, analytical standard
Supelco
p-Cymene, analytical standard
Supelco
Benzo[a]pyrene solution, 100 μg/mL in cyclohexane, analytical standard
Sigma-Aldrich
1-Methyl-2-pyrrolidinone, ReagentPlus®, 99%
Sigma-Aldrich
Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, meets USP testing specifications
Sigma-Aldrich
Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, ACS reagent