M2128

Thiazolyl Blue Tetrazolium Bromide

98%, powder

• Synonyma: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
• Empirický vzorec (Hillův zápis): C₁₈H₁₆BrN₅S
• Číslo CAS: 298-93-1
• Molekulová hmotnost: 414.32
• Beilstein/REAXYS Number: 4081397
• EC Number: 206-069-5
• MDL number: MFCD00011964
• UNSPSC Code: 12352111
• PubChem Substance ID: 24896603
• NACRES: NA.47

Vlastnosti

• product name: Thiazolyl Blue Tetrazolium Bromide, 98%
• assay: 98%
• form: powder
• color: dark yellow
• mp: 195 °C (dec.) (lit.)
• solubility: 2-methoxyethanol: soluble 20 mg/mL; ethanol: soluble 20 mg/mL; H₂O: 5 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Popis

Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:

• Proliferation assay.
• TNF (tumor necrosis factor) bioassay.

Bezpečnostní informace

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H315,H319,H335,H341
• pcodes: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)