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WTA2

Sigma-Aldrich

Complete Whole Transcriptome Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Synonyma:

transcriptome amplification kit

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Vybrat velikost

10 REACTIONS
22 800,00 Kč
50 REACTIONS
122 800,00 Kč

22 800,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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Vybrat velikost

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10 REACTIONS
22 800,00 Kč
50 REACTIONS
122 800,00 Kč

About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

22 800,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Quality Level

200

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

General description

WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library.

Application

Complete Whole Transcriptome Amplification Kit is used for the following applications:
  • To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.[1]
  • Reverse transcription and cDNA amplification[2][3][4][5][6][7]
  • For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles[8]
  • Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)[9]
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Features and Benefits

  • Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
  • Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
  • Contains all needed components for cDNA amplification
  • Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
  • Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism

Principle

The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product.

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list
  • Library Synthesis Enzyme
  • Library Synthesis Solution
  • Amplification Mix
  • Library Synthesis Buffer
  • W4502Water, Nuclease-Free Water, for Molecular BiologyBezpečností list
  • Amplification Enzyme
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology ReagentBezpečností list

related product

Č. produktu
Popis
Stanovení ceny

Storage Class

10 - Combustible liquids

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
SLCQ5847
SLCP9475
SLCQ8573
SLCQ8820
SLCP6950

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Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

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Navštívit knihovnu dokumentů

Jana Sachsenröder et al.
PloS one, 9(2), e88888-e88888 (2014-03-04)
The pig faecal virome, which comprises the community of viruses present in pig faeces, is complex and consists of pig viruses, bacteriophages, transiently passaged plant viruses and other minor virus species. Only little is known about factors influencing its general
Bert Vanmechelen et al.
BMC genomics, 19(1), 617-617 (2018-08-18)
In the past decade, many new paramyxoviruses that do not belong to any of the seven established genera in the family Paramyxoviridae have been discovered. Amongst them are J-virus (JPV), Beilong virus (BeiPV) and Tailam virus (TlmPV), three paramyxovirus species
137 differential gene expression of in vitro-matured bovine oocytes with or without a polar body.
M. M. Pereira et al.
Reproduction, Fertility, and Development, 24(1) , 181-181 (2011)
Richard J Hall et al.
Journal of virological methods, 195, 194-204 (2013-09-17)
The discovery of new or divergent viruses using metagenomics and high-throughput sequencing has become more commonplace. The preparation of a sample is known to have an effect on the representation of virus sequences within the metagenomic dataset yet comparatively little
A Bal et al.
BMC infectious diseases, 18(1), 537-537 (2018-10-31)
In recent years, metagenomic Next-Generation Sequencing (mNGS) has increasingly been used for an accurate assumption-free virological diagnosis. However, the systematic workflow evaluation on clinical respiratory samples and implementation of quality controls (QCs) is still lacking. A total of 3 QCs
Zobrazit všechny související dokumenty

Sortimentní položky

WTA2 Amplicon Modification for NGS

Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

Protokoly

Integration of Sigma® TransPlex® with Agilent Microarray

TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Související obsah

Whole Transcriptome Amplification FAQs

Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

Questions

  1. What are the differences between WTA1 and WTA2?

    1 answer

    1. WTA2 is designed to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Here are the main differences between the two kits:

      1. WTA1 was developed by Rubicon. WTA2 was developed by Sigma-Aldrich.
      2. The library synthesis primers for WTA2 are different and can prime more frequently, which is crucial for degraded RNAs.
      3. The library synthesis conditions are different due to the change in the primers.
      4. There are improved cycling conditions in WTA2 to optimize amplification of both high AT templates and high GC templates.
      5. WTA1 does not contain an amplification enzyme, which needs to be supplied separately. WTA2 includes the amplification enzyme.

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