RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Synonyma:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
41116158
NACRES:
NA.54
Doporučené produkty
General description
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Application
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Součásti soupravy, které jsou dostupné také samostatně
Č. produktu
Popis
Bezpečností list
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderBezpečností list
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderBezpečností list
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionBezpečností list
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionBezpečností list
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLBezpečností list
recommended
Č. produktu
Popis
Stanovení ceny
required but not provided
Č. produktu
Popis
Stanovení ceny
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Storage Class
3 - Flammable liquids
wgk_germany
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
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Sortimentní položky
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Protokoly
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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