Product D2886 - DNA Ligase from T4-infected Escherichia coli is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.
Unfortuntely, the reaction buffer is not offered. This enzyme cuts in many buffers including buffers for restriction enzymes. In order to work the enzyme needs 1mM ATP added to the ligation reaction.
A recipe for a standard T4 ligation buffer is as follows:
50 mM Tris-HCl, pH 7.8 (T2569)
10 mM MgCl2 (M1028)
10 mM dithiothreitol (D9779)
1 mM ATP (A7699)
25 ug/ml bovine serum albumin (B4287)
D2886
DNA Ligase from T4-infected Escherichia coli
buffered aqueous glycerol solution
Sinonimo/i:
Polydeoxyribonucleotide Synthase, Polynucleotide Ligase, T4 DNA Ligase
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100 UNITS
CHF 113.00
500 UNITS
CHF 374.00
CHF 113.00
Spedizione prevista il27 marzo 2025
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Cambia visualizzazione
100 UNITS
CHF 113.00
500 UNITS
CHF 374.00
About This Item
CHF 113.00
Spedizione prevista il27 marzo 2025
Prodotti consigliati
Grado
for molecular biology
Livello qualitativo
Stato
buffered aqueous glycerol solution
Attività specifica
4,000 U/mL
PM
68 kDa
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
bacteriophage T4 ... 30(1258680)
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Categorie correlate
Applicazioni
Suitable for:
- Ligation of blunt ended or cohesive DNA fragments
- Ligation of cloning vector and restriction insert fragments
- Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids
- Couple RNA single strands by bridging oligonucleotide adapters
Azioni biochim/fisiol
T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor.[1] Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture.[2] The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.[3]
Componenti
T4 DNA Ligase is supplied in a solution containing 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.
Definizione di unità
One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C.
Altre note
T4 DNA Ligase is inactivated by heating at 65 °C for 10 minutes.
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N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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