T1428

apo-Transferrin bovine

BioReagent, suitable for cell culture, ≥98%

• Synonym(e): Human serotransferrin
• CAS-Nummer: 11096-37-0
• EG-Nummer: 234-318-8
• MDL-Nummer: MFCD00130536
• UNSPSC-Code: 12352202
• NACRES: NA.83

Eigenschaften

• Biologische Quelle: bovine
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Assay: ≥98%
• Form: powder
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: iron, essentially free
• Löslichkeit: H₂O: 10 mg/mL
• UniProt-Hinterlegungsnummer: Q29443
• Lagertemp.: 2-8°C
• Angaben zum Gen: bovine ... TF(280705)

Beschreibung

Allgemeine Beschreibung

apo-Transferrin is an iron-free form of transferrin that is present in circulation.[1]

Anwendung

All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.

apo-Transferrin bovine has been used as a component of:

• basal medium for culturing bovine mammary epithelial cells[2][3]
• minimum essential medium α (MEM-α) for spermatogonial stem cells (SSCs) culture[4]
• induction medium for mesenchymal stem cell culture[5]

Biochem./physiol. Wirkung

apo-Transferrin (apoTf) aids in preventing iron accumulation in tissues, especially in non-transfusion dependent β-thalassemia.[6]

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)