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I9278

Sigma-Aldrich

Insulin -Lösung human

sterile-filtered, BioXtra, suitable for cell culture

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About This Item

CAS-Nummer:
11061-68-0
MDL-Nummer:
MFCD00131380
UNSPSC-Code:
12352209
NACRES:
NA.77
Form:
solution
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Sterilität

sterile-filtered

Qualitätsniveau

400

Produktlinie

BioXtra

Form

solution

Konzentration

9.5-11.5 mg/mL

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤50 EU/mL endotoxin

pH-Wert

7.9-8.5

UniProt-Hinterlegungsnummer

P01308

Lagertemp.

2-8°C

InChIKey

PBGKTOXHQIOBKM-FHFVDXKLSA-N

Angaben zum Gen

human ... INS(3630)

Allgemeine Beschreibung

Insulin ist ein zweikettiges Polypeptidhormon, das von den β-Zellen der Pankreasinseln gebildet wird. Sein Molekulargewicht beträgt ca. 5800 kDa. Die α- und β-Ketten sind durch zwei Disulfidbrücken verbunden. Die α-Kette enthält eine einzelne Disulfidbindung.

Anwendung

Humane Insulinlösung wird zu 0,5 bis 1 ml je Liter Medium zur Verwendung in Zellkulturanwendungen empfohlen.
Humane Insulinlösung wird für Insulintoleranztests bei Mäusen und Ratten verwendet.[1][2] Es wird auch zur adipogenen Differenzierung von aus humanen embryonalen Stammzellen (hESC) gewonnenen Auswuchskulturen und hMSC (humane mesenchymale Stromazellen) verwendet.[3][4]
Humaninsulinlösung wird als Zusatz in Dulbecco′ modifiziertem Eagle-Medium′ (DMEM) zur Kultivierung der Epithel- und Endometriumzelllinie vom Menschen, Minimal Essential Medium zur Kultivierung der Brust-Adenokarzinom-Zelllinie vom Menschen und Medium 199 zur Kultivierung einer normalen Ovarialzelllinie eingesetzt.

Biochem./physiol. Wirkung

Insulin spielt eine Rolle in verschiedenen zellulären Prozessen, z. B. bei der Förderung des Zellwachstums, der Regulierung des Zellzyklus und der Förderung der Glucoseaufnahme. Es wird häufig als Proteinsupplement in serumfreien Nährmedien für Säugetierzellen verwendet, um das optimale Wachstum und die Erhaltung der Säugetierzellen zu fördern.

Sonstige Hinweise

InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1

Physikalische Form

Stabil bei 2 bis 8 °C

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000341617
0000327021
0000341617
SLCQ6396
SLCN8981

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Die Dokumentenbibliothek aufrufen

E Comisso et al.
Oncogene, 36(30), 4253-4266 (2017-03-21)
OCT4 (Octamer-binding transcription factor 4) is essential for embryonic stem cell self-renewal. Here we show that OCT4 increases the aggressiveness of high-grade serous ovarian cancer (HG-SOC) by inactivating the Retinoblastoma tumor suppressor pathway and enhancing mitotic stability in cancer cells.
Farinaz Ghodrati et al.
Scientific reports, 8(1), 8654-8654 (2018-06-07)
At times, it can be difficult to discern if a lack of overlap in reported interactions for a protein-of-interest reflects differences in methodology or biology. In such instances, systematic analyses of protein-protein networks across diverse paradigms can provide valuable insights.
Early cardiac changes in a rat model of prediabetes: brain natriuretic peptide overexpression seems to be the best marker.
Nunes S, et al.
Cardiovascular Diabetology, 12, 44-44 (2013)
Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.
Jones M et al.
Cytotherapy, 15, 1323-1323 (2013)
Amer Mahmood et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 25(6), 1216-1233 (2010-03-05)
Directing differentiation of human embryonic stem cells (hESCs) into specific cell types using an easy and reproducible protocol is a prerequisite for the clinical use of hESCs in regenerative-medicine procedures. Here, we report a protocol for directing the differentiation of
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Questions

1–10 of 15 Questions  

  1. How is this insulin produced? In E.Coli? Yeast?...?

    1 answer

    1. This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.

      Helpful?

  2. Is this product suitable for insulin tolerance tests in mice

    1 answer

    1. This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/

      Helpful?

  3. How long does the insulin remain potent for if stored in the fridge?

    1 answer

    1. This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/i9278#product-documentation

      Helpful?

  4. Is this insulin considered of fast or slow action?

    1 answer

    1. This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
      Insulin Glargine - Slow acting Insulin
      https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=product

      Aspart Insulin - Fast acting Insulin
      https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=product

      Lispro Insulin - Fast acting Insulin
      https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=product

      Helpful?

  5. Is this human insulin suitable for use in mouse cell culture?

    1 answer

    1. Yes, this product is suitable for mouse cell culture and has been cell culture tested on the L929 and 3T3 mouse cell lines. Please see the link below to review peer-reviewed articles that may be of interest:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892261/#:~:text=This%20process%20requires%20DRAM%2D1,and%20permitting%20efficient%20mTORC1%20activation.

      Helpful?

  6. what is the morality of the product I9278, Insulin solution human?

    1 answer

    1. The molarity of a 10 mg/mL solution of human insulin, with a molecular weight of 5807 daltons, is approximately 1.7 μmol/mL or 1.7 mM.

      Helpful?

  7. What kind of solution is the insulin (Product number: I9278) dissolved in?

    1 answer

    1. The insulin (cat# I9278) is dissolved in HEPES solution.

      Helpful?

  8. Are there any contaminants (IgG, IgM example) in I9278?

    1 answer

    1. Unfortunately, this product is not tested for impurities.

      Helpful?

  9. What is the content of the insulin solution/buffer (any perservatives and non insulin components?)

    1 answer

    1. This product is prepared in a HEPES buffer, pH 7.9 - 8.5.

      Helpful?

  10. Can you store this product aliquoted at -20 degrees to extend shelf-life?

    1 answer

    1. For research applications, freezing insulin solution has been a common pratice. However, solutions available on the commercial market for therapeutic use are never frozen, in order to maintain maximum activity. There have been no stability studies perform on the shelf life of frozen insulin solutions. Please see the link below to review a publication that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721649/

      Helpful?

1–10 of 15 Questions  

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