P9172

Pyronin Y

for NA electrophoresis

• Synonym(e): Pyronin G, Pyronin Y, Pyronin J
• Empirische Formel (Hill-System): C₁₇H₁₉ClN₂O
• CAS-Nummer: 92-32-0
• Molekulargewicht: 302.80
• Farbindexnummer: 45005
• Beilstein: 3795789
• EG-Nummer: 202-147-8
• MDL-Nummer: MFCD00011725
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 57654569
• NACRES: NA.52

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Form: powder
• Zusammensetzung: Dye content, ≥45%
• Methode(n): nucleic acid detection: suitable
• mp (Schmelzpunkt): 250-260 °C (lit.)
• Löslichkeit: water: 10 mg/mL, dark red
• Eignung: suitable for nucleic acid staining
• Lagertemp.: room temp
• SMILES String: [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
• InChI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• InChIKey: INCIMLINXXICKS-UHFFFAOYSA-M

Beschreibung

Anwendung

Pyronin Y has been used:

• in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
• as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
• staining of CD34⁺ cells

Biochem./physiol. Wirkung

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Angaben zur Herstellung

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)