This product has not been specifically tested for suitability in SCSA and sperm DNA structure assays. However, there are a number of publications that have used this product for such applications. A thorough review of the literature is recommended. Please see the link below to review one such publication:
https://www.sciencedirect.com/science/article/pii/S0188440901003289
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A6014
Acridinorange Hemi-(zinkchlorid) (Salz)
For nucleic acid staining in cells or gels
Synonym(e):
3,6-Bis-(dimethylamino)-acridin-hydrochlorid Zinkchlorid Doppelsalz, 3,6-Bis-(dimethylamino)-acridinium-chlorid Hemi-(zinkchlorid) (Salz), Basic Orange 14
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10 G
CHF 95.80
25 G
CHF 222.00
100 G
CHF 529.00
CHF 95.80
Voraussichtliches Versanddatum01. Juni 2025
Alle Fotos(2)
Größe auswählen
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10 G
CHF 95.80
25 G
CHF 222.00
100 G
CHF 529.00
About This Item
Lineare Formel:
C17H20ClN3 · HCl · 1/2ZnCl2
CAS-Nummer:
Molekulargewicht:
369.96
Farbindexnummer:
46005
Beilstein:
3734978
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.52
CHF 95.80
Voraussichtliches Versanddatum01. Juni 2025
Empfohlene Produkte
Qualitätsniveau
Produktlinie
BioReagent
Form
powder
Zusammensetzung
Dye content, ~80%
Methode(n)
nucleic acid detection: suitable
Löslichkeit
ethanol: 2 mg/mL
4 mg/mL (2-methoxyethanol (EGME))
water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
Eignung
suitable for flow cytometry
suitable for microscopy
Lagertemp.
room temp
SMILES String
Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
InChI
1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
InChIKey
RAHGLSRJKRXOSY-UHFFFAOYSA-L
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Allgemeine Beschreibung
Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.
Anwendung
Acridinorange, ein zelldurchdringender metachromatischer fluoreszierender kationischer Farbstoff, der DNA und RNA interkaliert, wird in der Fluoreszenz- und Epifluoreszenzmikroskopie verwendet. Der Acridinorange-Farbstoff wurde verwendet, um den Mitochondrien- und Lysosomen-Gehalt mittels Durchflusszytometrie zu analysieren, um Multidrug-Resistenz zu charakterisieren und um Veränderungen in der mitochondrialen Masse während der Apoptose in Ratten-Thymozyten zu messen.
Suitable for
- detection of nucleic acids separated by gel electrophoresis
- fluorescence and epifluorescence microscopy
- analysis of mitochondria and lysosomes by flow cytometry
- DNA staining in apoptosis studies
Leistungsmerkmale und Vorteile
- 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
- differential staining of single- and double-stranded polynucleotides
Prinzip
Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.[1]
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Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Muta. 2
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Z Darzynkiewicz et al.
Cytometry, 13(8), 795-808 (1992-01-01)
The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. Most of these methods were applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA
H Baisch et al.
Cell proliferation, 32(5), 303-319 (2000-01-05)
Early indicators of apoptosis in mammalian cells are membrane potential breakdown (loss) in mitochondria (MPLM), chromatin condensation, DNA degradation, and phosphatidylserine exposure (PSE) on the outside plasma membrane. One aim of the present study was to determine the kinetics of
F Durrieu et al.
Cytometry, 36(2), 140-149 (1999-11-30)
Some forms of chemoresistance in leukemia may start from failure of tumour cells to successfully undergo apoptosis and Bcl-2 may play a role in this defect. Therefore, we evaluated the Bcl-2 content and synthesis in relation with the apoptotic potential
G K McMaster et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(11), 4835-4838 (1977-11-01)
We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The
Yu Sato et al.
Anatomical science international, 94(2), 199-208 (2019-01-03)
Neurons are classified into several morphological types according to the locations of their somata and the branching patterns of their axons and dendrites. Recent studies suggest that these morphological features are related to their physiological properties, including firing characteristics, responses
Artikel
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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Is product A6014, Acridine Orange hemi(zinc chloride) salt, used for sperm chromatin structure assay (SCSA) and sperm DNA structure assay?
1 answer-
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