The product has not been validated for immunofluorescence assay in our laboratory to establish a reference protocol. In cases where propidium iodide binds to both DNA and RNA, the use of RNase is common to differentiate DNA staining from undesired RNA staining. For an application protocol, one may refer to a citation of this product in an immunofluorescence assay that utilizes RNase to visualize DNA-specific staining. The citation is as follows: J Cancer. 2020 Mar 5;11(11):3274-3287. The article discusses the dual role of BI 2536, a small-molecule inhibitor targeting PLK1, in inducing apoptosis and attenuating autophagy in neuroblastoma cells. The PMID for the article is 32231733.
Alle Fotos(9)
Wichtige Dokumente
P4170
Propidiumjodid
≥94.0% (HPLC)
Synonym(e):
3,8-Diamino-5-(3-diethylaminopropyl)-6-phenyl-phenanthridiniumiodid-methiodid
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
10 MG
CHF 76.00
25 MG
CHF 115.00
100 MG
CHF 376.00
250 MG
CHF 475.00
500 MG
CHF 788.00
1 G
CHF 1’680.00
Alle Fotos(9)
Größe auswählen
Ansicht ändern
10 MG
CHF 76.00
25 MG
CHF 115.00
100 MG
CHF 376.00
250 MG
CHF 475.00
500 MG
CHF 788.00
1 G
CHF 1’680.00
About This Item
Empirische Formel (Hill-System):
C27H34I2N4
CAS-Nummer:
Molekulargewicht:
668.39
Beilstein:
3843838
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
41106305
PubChem Substanz-ID:
NACRES:
NA.77
Empfohlene Produkte
Qualitätsniveau
Assay
≥94.0% (HPLC)
Form
powder
mp (Schmelzpunkt)
220-225 °C (dec.) (lit.)
Lagertemp.
2-8°C
SMILES String
[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChIKey
XJMOSONTPMZWPB-UHFFFAOYSA-M
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Allgemeine Beschreibung
Propidiumiodid (PI) ist ein fluoreszierender Farbstoff, derzur Färbung von Nukleinsäuren verwendet werden kann.[1] Es kann nicht in lebensfähige oderintakte Zellen eindringen. Er kann leicht in abgestorbene und sterbende Zellen sowie Zellen mit beeinträchtigter Membran eindringen.[1][2] PI bindet leicht an DNA.[3]
Anwendung
Leistungsmerkmale und Vorteile
Diese Verbindung ist ein wichtiges Produkt für die Forschung im Bereich der Apoptose. Weitere empfohlene Apoptoseprodukte finden Sie hier Weitere Informationen über bioaktive kleine Moleküle für andere Forschungsbereiche finden Sie hier: sigma.com/discover-bsm.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Muta. 2
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Gloves
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Julia Burrill et al.
Forensic science international. Genetics, 46, 102269-102269 (2020-03-11)
Forensic DNA typing from touched or handled items in routine casework is increasing as the sensitivity of detection techniques improves. Our understanding of the cellular/acellular content of touch deposits and the origins of the DNA therein is still limited. This
Aswathy Shailaja et al.
Biofilm, 4, 100090-100090 (2022-11-18)
Filamentous fungi are ubiquitous and frequent components of biofilms. A means to visualize them and quantify their viability is essential for understanding their development and disruption. However, quantifying filamentous fungal biofilms poses challenges because, unlike yeasts and bacteria, they are
Robin Kumar et al.
Methods in molecular biology (Clifton, N.J.), 2279, 213-223 (2021-03-09)
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca2+ ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from
Haiying Liu et al.
Frontiers in cell and developmental biology, 9, 684857-684857 (2021-10-05)
It has been reported that calpain/caspase-mediated apoptosis induced by endoplasmic reticulum stress (ERS) in hepatic stellate cells (HSCs) by previous studies. At present, the activation of HSC is an important cause of liver fibrosis, and the induction of HSC apoptosis
Wei Liang et al.
Molecular immunology, 112, 312-321 (2019-06-24)
Precise glycosylation plays a crucial and distinctive role in thymic T cell development. The core fucosylation is dramatically up-regulated at the transition from CD4-CD8- (DN) to CD4+CD8+ (DP) in the thymic development. Ablation of core fucosylation in T cells did
Artikel
Cell cycle phases (G1, S, G2, M) regulate cell growth, DNA replication, and division in proliferating cells.
Apoptosis regulation involves multiple pathways and molecules for cellular homeostasis.
Verwandter Inhalt
Nancy-520 for DNA Detection and Quantitation
-
Is it necessary to carry out an RNase treatment before staining with propidium iodide in my immunofluorescence assay on insect tissue fixed in 4% paraformaldehyde? Additionally, is there the complete staining protocol for propidium iodide in immunofluorescence using a confocal microscope?
1 answer-
Helpful?
-
-
What is the procedure for dissolving this product?
1 answer-
Propidium iodide is soluble in water at a concentration of 1 mg/mL.
Helpful?
-
-
Is this reagent suited for viability assays at FACS?
1 answer-
While this product is not tested for any specific application, propidium iodide is commonly used in cell-based assays such as FACS. Please see the link below to review publications detailing the use of this item in flow cytometry:
https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=%22p4170%22+flow+cytometry&btnG=Helpful?
-
-
What is the method to dissolve this product?
1 answer-
Propidium iodide can be dissolved in water at a concentration of 1 mg/mL. Please see the sample Certificate of Analysis for solubility information: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4170-BULKWXBF1544V.pdf
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
