CGD1

Cell Growth Determination Kit, MTT based

• Synonym(e): Mitochondrial activity assay
• UNSPSC-Code: 12352207
• NACRES: NA.75

Eigenschaften

• Verpackung: pkg of 1 kit
• Methode(n): UV/Vis spectroscopy: suitable; protein quantification: suitable
• Amax: 570 nm
• Anwendung(en): cell analysis; detection
• Nachweisverfahren: colorimetric
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Anwendung

Cell Growth Determination Kit, MTT based has been used:

• to measure the cell viability of preosteoblastic cells
• in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
• to determine the cell viability of primary cervical epithelial cells

Verpackung

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

Sicherheitshinweise

• Piktogramme: GHS02,GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H225,H319,H336,H341
• P-Sätze: P202 - P210 - P233 - P240 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
• Zielorgane: Central nervous system
• Lagerklassenschlüssel: 3 - Flammable liquids
• Flammpunkt (°F): 53.6 °F - closed cup
• Flammpunkt (°C): 12 °C - closed cup