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Merck

A5278

Sigma-Aldrich

Anti-Maus-IgG (Gesamtmolekül)-Peroxidase in Ziege hergestellte Antikörper

IgG fraction of antiserum, buffered aqueous solution

Synonym(e):

Anti Mouse Hrp Sigma

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Konjugat

peroxidase conjugate

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Methode(n)

direct ELISA: 1:10,000

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid. These antibodies are capable of fighting bacterial and viral infection. They also regulate several functions like placental transport, cell surface-receptor binding and complement activation. Anti-mouse IgG (whole molecule)-peroxidase antibody can be used in ELISA (diluted 1: 200 in PBST) . It can also be used in western blotting. Goat anti-mouse IgG-peroxidase antibody reacts specifically with mouse IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM myeloma proteins.

Immunogen

Purified mouse IgG.

Anwendung

Anti-mouse IgG (whole molecule)-peroxidase antibody (diluted 1: 300) can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.
Western blot analysis using HRP conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:3000 in BSA/PBS was performed to detect IgG antibodies against outer-membrane vexicle proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Angaben zur Herstellung

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

Exclamation markEnvironment

Signalwort

Warning

Gefahreneinstufungen

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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M Tojo et al.
Clinical chemistry, 34(3), 539-543 (1988-03-01)
Hybridomas obtained by fusing the spleen cells of BALB/c female mice hyperimmunized with heat-killed yeast-form cells of Candida albicans NIH B-792 strain and a mouse myeloma cell line, P3X63Ag8.653, produced antibodies to beta-1,2-linked oligomannosyl residues in the phosphomannan-protein complex of
Sabine Hombach-Klonisch et al.
Toxicological sciences : an official journal of the Society of Toxicology, 90(2), 519-528 (2006-01-25)
Oviduct epithelial cells are important for the nourishment and survival of ovulated oocytes and early embryos, and they respond to the steroid hormones estrogen and progesterone. Endocrine-disrupting polyhalogenated aromatic hydrocarbons (PHAH) are environmental toxins that act in part through the
Lucila O Fukasawa et al.
Journal of medical microbiology, 52(Pt 2), 121-125 (2003-01-25)
A mAb against the NadA protein from Neisseria meningitidis strain 3006 (serosubtype B : 2b : P1.2 : P5.2,8) demonstrated strong bactericidal activity against Brazilian epidemic serogroup B strain N44/89 (B : 4,7 : P1.19,15 : P5.5,7) and a serogroup
Fabian M Arnold et al.
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Molecular research on mycobacteria relies on a multitude of tools for the genetic manipulation of these clinically important bacteria. However, a uniform set of vectors allowing for standardized cloning procedures is not available. Here, we developed a versatile series of
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Endocrinology, 155(7), 2391-2401 (2014-05-16)
Mutations in the PCSK1 gene encoding prohormone convertase 1/3 (PC1/3) are strongly associated with obesity in humans. The PC1/3(N222D) mutant mouse thus far represents the only mouse model that mimics the PC1/3 obesity phenotype in humans. The present investigation addresses

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