H8032

Bouillon nach Hanahan (SOB-Medium)

powder microbial growth medium

• Synonym(e): SOB Medium
• UNSPSC-Code: 41106200
• NACRES: NA.85

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 100
• Beschreibung: recommended for cultivation of recombinant E. coli.
• Sterilität: non-sterile
• Form: powder
• Zusammensetzung: KCl, 0.186 g/L ; MgSO₄, 2.4 g/L ; NaCl, 0.5 g/L ; Tryptone, 20 g/L ; Yeast extract, 5 g/L
• Methode(n): microbiological culture: suitable
• pH-Wert: 6.8-7.2(2.8% solution)
• Anwendung(en): food and beverages; microbiology
• Lagertemp.: room temp
• Eignung: nonselective for Escherichia coli; nonselective for coliforms

Beschreibung

Allgemeine Beschreibung

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.

Anwendung

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Leistungsmerkmale und Vorteile

Hannahan′s Broth (SOB) powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Rekonstituierung

Herstellung
1. 28.09 g in 1 L destillertem Wasser suspendieren.
2. Für 15 Minuten bei 121°C autoklavieren.

Sonstige Hinweise

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves