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S1797

Sigma-Aldrich

SOC-Medium

For use in transformation

Synonym(e):

Super Optimal broth

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About This Item

UNSPSC-Code:
41106200
NACRES:
NA.85
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Qualität

Molecular Biology
for molecular biology

Sterilität

0.2 μm filtered

Form

liquid

Zusammensetzung

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

Methode(n)

microbiological culture: suitable

Anwendung(en)

agriculture

Lagertemp.

2-8°C

Eignung

nonselective for Escherichia coli
nonselective for coliforms

Verwandte Kategorien

Allgemeine Beschreibung

SOC-Medium (superoptimales Medium mit katabolischem Repressor) ist ein glucosereiches mikrobielles Wachstumsmedium, das primär im Rückgewinnungsschritt bei der Transformation von kompetenten Escherichia-coli-Zellen verwendet wird. Die Kultivierung von E.-coli-Zellen in dieser nährstoffreichen mikrobiellen Bouillon erhöht die Transformationseffizienz rekombinanter Plasmide, die toxische Proteingene enthalten, indem sie die unbeabsichtigte Aktivierung des lac-Promotors auf dem Klonierungsvektor hemmt.[1] SOC-Medium enthält Peptide, Aminosäuren, wasserlösliche Vitamine und Glucose in einer Formulierung mit niedrigem Salzgehalt.

Anwendung

SOC-Medium wird für die Transformation von DH5α-kompetenten E.-coli-Zellen verwendet. [2] Außerdem wird es zur Kultivierung weißer Kolonien nach dem Klonen zur Identifizierung des zu untersuchenden rekombinanten Proteins verwendet. [3]

Leistungsmerkmale und Vorteile

Die SOC-Flüssigkeit bietet Folgendes:
  • Gefiltertes und sterilisiertes gebrauchsfertiges Format
  • Praktische Packungsgrößen
  • Standardmäßige Formulierung

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
SLCN8706
SLCL9800
SLCN2539
SLCF7416
SLCG7766

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Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
Alle zugehörigen Dokumente anzeigen

Artikel

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protokolle zur Bakterientransformation

Allgemeine Protokolle zum Wachstum kompetenter Zellen und ihrer Transformation (Aufnahme von DNA).

Protokolle

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Questions

1–4 of 4 Questions  

  1. That is the shelf life of SOC medium if kept refrigerated at 4C?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Why is SOC Medium (S1797) used to recover bacterial cells before transformation?

    1 answer

    1. SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.

      Helpful?

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