Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Principaux documents

Voir toute la documentation

C0412

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonyme(s) :

Con A

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

5 MG
87,30 $

87,30 $

Disponible pour expédition le09 avril 2025Détails

Toutes les photos(1)

Sélectionner une taille de conditionnement

Changer de vue
5 MG
87,30 $

About This Item

Numéro CAS:
11028-71-0
Numéro CE :
234-258-2
Numéro MDL:
MFCD00071069
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.32

87,30 $

Disponible pour expédition le09 avril 2025Détails

Niveau de qualité

200

Stérilité

γ-irradiated

Type

Type IV-S

Gamme de produits

BioReagent

Forme

lyophilized powder

Technique(s)

cell culture | mammalian: suitable

Impuretés

salt, essentially free

Solubilité

PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Application

Concanavalin A from Canavalia ensiformis (jack bean) has been used:
  • to induce spleen blastogenesis[1]
  • to generate ConA blast targets from B6 and lpr spleen cells[2]
  • to stimulate splenic lymphocytes from estrogen-and placebo-treated mice[3]

Actions biochimiques/physiologiques

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Remarque sur l'analyse

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334,H361

Classification des risques

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCP7735
SLCB0143
SLBT4976
SLBN5209V
SLBJ2854V

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Chengfang Pang et al.
Toxicology letters, 311, 49-57 (2019-04-25)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener of dioxins, is a persistent and ubiquitous environmental contaminant. Although the immunotoxic effects of TCDD have been reported, the mechanisms underlying these effects are still unclear. In this study, we have determined the toxic
Immune responsiveness of splenocytes after chronic daily melatonin administration in male Syrian hamsters
Champney TH, et al.
Immunology Letters, 58(2), 95-100 (1997)
CD8+ T cell tolerance to a tumor-associated antigen is maintained at the level of expansion rather than effector function
Ohlen C, et al.
The Journal of Experimental Medicine, 195(11), 1407-1418 (2002)
17 beta-Estradiol downregulates interferon regulatory factor-1 in murine splenocytes
Lengi AJ, et al.
Journal of Molecular Endocrinology, 37(3), 421-432 (2006)
Chengfang Pang et al.
Toxicology letters, 311, 49-57 (2019-04-25)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener of dioxins, is a persistent and ubiquitous environmental contaminant. Although the immunotoxic effects of TCDD have been reported, the mechanisms underlying these effects are still unclear. In this study, we have determined the toxic
Afficher tous les articles scientifiques apparentés

Questions

  1. ConA 제품 (C0412)에 관해 질문 드립니다. ConA가 Ca2+, Mn2+ 이온이 존재해야 활성화가 된다고 하는데 해당 제품은 powder형태이며 이는 따로 용액이 필요합니다. 그렇다면 이 제품 안에 저 금속 이온이 함께 들어있는 건지, 아니면 Sigma 제품 중에 이 제품을 혼합할 수 있는 PBS가 따로 있는지(있다면 카탈로그 번호 알려주셨으면 합니다) 궁금합니다.

    1 answer

    1. Concanavalin A has 2 metal binding sites per monomer. Calcium and Manganese are required to bind these sites in order for sugar binding to occur. It is recommended to prepare stock solutions in PBS containing Ca2+ and Mn2+.

      See the link below to the product datasheet which includes preparation instructions:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/331/341/c0412dat.pdf

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique