This product is soluble in water at a concentration of 2 mg/ml, resulting in a clear solution. Concanavalin A from Canavalia ensiformis (Jack bean) has been utilized in the colabeling of Fischer rat thyroid epithelial cells, to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice, and to detect glycoproteins containing α-D-mannose and β-D-glucose when used with an avidin or streptavidin detection system. This product has not been tested or validated for immunoprecipitation experiments. It is recommended to consult literature for procedures to isolate plasma membrane surface proteins using Concanavalin A.
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
2 mg | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 154,00 $ |
10 mg | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 216,00 $ |
A propos de cet article
154,00 $
biological source
Canavalia ensiformis
Quality Segment
conjugate
biotin conjugate
type
Type IV
form
lyophilized powder
composition
Protein, ~85% ε1%/280
extent of labeling
4-8 mol biotin per mol protein
impurities
Carbohydrate, essentially free
storage temp.
2-8°C
General description
Application
- in the colabelling of fischer rat thyroid (FRT) epithelia(104)
- to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105)
- to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)
Biochem/physiol Actions
Packaging
Physical form
Analysis Note
1 of 1
Cet article | |||
|---|---|---|---|
| biological source Canavalia ensiformis | biological source - | biological source Canavalia ensiformis | biological source Canavalia ensiformis |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form saline suspension |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| impurities Carbohydrate, essentially free | impurities Carbohydrate, essentially free, salt, essentially free | impurities Carbohydrate, essentially free | impurities - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| conjugate biotin conjugate | conjugate - | conjugate FITC conjugate | conjugate agarose conjugate |
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What agent should be used to resolve conA powder? Is this type of conA suitable for isolating the proteins on the surface of the plasma membrane? What concentration of conA should be used in IP experiments for isolating plasma membrane surface proteins?
1 answer-
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What solvent does this product dissolve in?
1 answer-
This product is soluble in water up to 10 mg/mL and may yield a slightly hazy, colorless to faint yellow solution. The solubility may be increased by the addition of sodium chloride.
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