If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
L2880
Lipopolysaccharides from Escherichia coli O55:B5
purified by phenol extraction
Synonyme(s) :
LPS
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About This Item
Produits recommandés
Source biologique
Escherichia coli (O55:B5)
Niveau de qualité
Forme
lyophilized powder
Produit purifié par
phenol extraction
Technique(s)
cell based assay: suitable
Impuretés
<3% Protein (Lowry)
Couleur
off-white to tan
Solubilité
water: soluble
Application(s)
cell analysis
clinical research
life science and biopharma
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
Application
- pour stimuler des hépatocytes et des cellules non parenchymateuses[4].
- pour stimuler la rate et étudier l'expression du gène Malitaf chez la carpe de Wuchang[5].
- dans le cadre du modèle d'hépatite fulminante induite par la concanavaline A (ConA) et le complexe D-galactosamine/lipopolysaccharide (D-GalN/LPS) pour étudier l'effet de l'hespérétine (composé naturel)[6].
- pour contrôler la viabilité de cellules souches mésenchymateuses dérivées de villosités choriales humaines (CSM-VCh) par un test au méthyl thiazolyl tétrazolium (MTT)[7].
- pour induire une toxicité vis-à-vis des oligodendrocytes afin d'évaluer les effets neuroprotecteurs de quelques composés[8].
- pour induire une sarcopénie chez des rats et évaluer les effets antioxydants d'une hydroxyapatite à surface modifiée par un traitement hydrophobe et chargée en curcumine.[9]
Actions biochimiques/physiologiques
Autres remarques
Produit(s) apparenté(s)
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 2 Oral
Code de la classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Articles
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Hi, Can you provide the information regarding molecular weight of this LPS?
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The molecular weight of this product has not been determined. In general, the molecular weight of native LPS may range from 1 – 4 million Daltons or higher. When treated with SDS and heat, the molecular weight of LPS may range from 50 to 100 kDa.
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What is the number of acetyl groups attached to the lipid A moiety?
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The acyl chain length and number of acyl groups may vary between bacterial species. Certain researchers claim there are 6 acyl groups for LPS from this strain of bacteria. Please refer to those peer reviewed papers below for more information.
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Can you provide information on the solubility and solution stability of this product?
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LPS products in solution are expected to be stable in phosphate buffered saline at a concentration of 1 mg/mL when stored at 4 °C for at least one month. For long-term storage, it is recommended to use frozen aliquots. In order to prevent LPS binding to glass, it is advisable to store the solution in silanized containers. If glass containers are used, it is recommended to vortex the solutions for at least 30 minutes to dissolve any bound LPS.
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Does the bacterial strain have specific information available, such as whether it originates from a private collection or if there is an associated ATCC number?
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Unfortunately, the specific strains used to make L2880 are considered proprietary.
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hello, I was wondering what the best way to reconstitute this LPS is
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LPS products can be reconstituted in phosphate buffered saline at 1 mg/mL.
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Which LPS grade should be used to induce neurotoxicity in rats?
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There is no Lipopolysaccharide product offered specifically for the induction of neurotoxicity in rats. Escherichia coli strain O55:B5 has been used to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of certain compounds. In addition, Escherichia coli strain O111:B4 has been cited in the literature as suitable for LPS-induced neurotoxicity. For use with animal models, LPS purified by gel filtration is most commonly used to eliminate the risk of any residual solvents.
Please see the links below to review the E. coli strains O111:B4 and O55:B5 that have been purified by gel filtration:
Lipopolysaccharides – Glycobiology
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides
Lipopolysaccharides – selection table
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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