HPLC Analysis of ¹⁵N-Acetylcysteine Enantiomers on Astec^® (R,R) P-CAP^™

column

Astec (R,R) P-CAP, 25 cm x 4.6 mm I.D., 5 μm particles (31024AST)

mobile phase

20 mM ammonium acetate in: (A) acetonitrile; (B) methanol; (70:30, A:B)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

1 μL

sample

5 mg/mL in methanol