Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Bio-medical materials and engineering (2014-09-18)

Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao

RESUMO

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

MATERIAIS

Número do produto

Marca

Descrição do produto

E7023

Sigma-Aldrich

Álcool etílico, puro, prova 200, Molecular Biology

459844

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Álcool etílico, puro, 200 proof, ACS reagent, ≥99.5%

34856

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Diclorometano, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

319902

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Acetato de etila, ACS reagent, ≥99.5%

459828

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Álcool etílico, puro, 200 proof

34858

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Acetato de etila, suitable for HPLC, ≥99.7%

D65100

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Diclorometano, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

650463

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Diclorometano, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer

493546

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Álcool etílico, puro, 200 proof, meets USP testing specifications

650528

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Acetato de etila, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%

270997

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Diclorometano, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

459836

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Álcool etílico, puro, 200 proof, anhydrous, ≥99.5%

471283

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Trietilamina, ≥99.5%

E7148

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Álcool etílico, puro, for molecular biology

130672

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N-Hydroxysuccinimide, 98%

493511

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T0886

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Trietilamina, ≥99%

32222

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Diclorometano, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)

02870

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Etanol, ACS reagent, prima fine spirit, without additive, F15 o¹

90335

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Trietilamina, BioUltra, ≥99.5% (GC)

320269

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Diclorometano, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

90340

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Trietilamina, puriss. p.a., ≥99.5% (GC)

51976

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Etanol, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

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Acetato de etila, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

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Acetato de etila, suitable for HPLC, ≥99.8%

270989

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02856

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Etanol, purum, absolute ethanol, denaturated with 4.8% isopropanol, A15 IPA¹, ≥99.8% (based on denaturant-free substance)

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Triphosgene, reagent grade, 98%

PHR1070

Supelco

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