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Y1003

Sigma-Aldrich

2x YT medium

Liquid microbial growth medium

Sinônimo(s):

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium

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About This Item

Código UNSPSC:
41106225
NACRES:
NA.85

grau

for molecular biology

esterilidade

sterile; 0.2 μm filtered

forma

liquid

técnica(s)

microbiological culture: suitable

pH

6.5-7.5

aplicação(ões)

microbiology

temperatura de armazenamento

room temp

adequação

nonselective for Escherichia coli
nonselective for coliforms

Categorias relacionadas

Descrição geral

2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Aplicação

2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Características e benefícios

2xYT liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Componentes

16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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