2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).
Y2377
2x YT medium
Powder microbial growth medium
Sinônimo(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
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250 G
R$ 341,00
About This Item
Código UNSPSC:
41106225
NACRES:
NA.85
grau
Molecular Biology
for molecular biology
esterilidade
non-sterile
Formulário
powder
técnica(s)
microbiological culture: suitable
pH
6.8-7.2(3.1% solution)
aplicação(ões)
food and beverages
microbiology
temperatura de armazenamento
room temp
adequação
nonselective for Escherichia coli
nonselective for coliforms
Descrição geral
2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Aplicação
2x YT medium has been used for culturing E. coli BL21 for cloning [1], E. coli SS320 cells for scaffold synthesis [2], and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens[3] and E. coli DH10B.[4]
Características e benefícios
2xYT powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Componentes
16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl
10 g/L Yeast Extract
5 g/L NaCl
Nota de preparo
1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
Reconstituição
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Rebecca R Du et al.
ACS nano, 16(12), 20340-20352 (2022-12-03)
Three-dimensional wireframe DNA origami have programmable structural and sequence features that render them potentially suitable for prophylactic and therapeutic applications. However, their innate immunological properties, which stem from parameters including geometric shape and cytosine-phosphate-guanine dinucleotide (CpG) content, remain largely unknown.
Bacterial artificial chromosomes: A functional genomics tool for the study of positive-strand RNA viruses
Yun SI, et al.
Journal of Visualized Experiments, 106, e53164-e53164 (2015)
Telomere-mediated Chromosomal Truncation via Agrobacterium tumefaciens or Particle Bombardment to Produce Engineered Minichromosomes in Plants
Graham ND, et al.
Bio-protocol, 5(18) (2015)
Designed active-site library reveals thousands of functional GFP variants
Weinstein J Y. et al.
Nature Communications (2023)
Basem Al-Shayeb et al.
Cell, 185(24), 4574-4586 (2022-11-25)
CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find that CRISPR systems are also encoded in diverse bacteriophages, where they occur as divergent and hypercompact anti-viral systems. Bacteriophage-encoded
Protocolos
General protocols for growth of competent cells in microbial medium.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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