When autoclaved or filter sterilized, the prepared solution may be stored tightly sealed at room temperature for up to 2 years.
M6030
M9, Minimal Salts, 5X
powder, minimal microbial growth medium
Sinônimo(s):
M9 media
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1 KG
R$ 990,00
R$ 990,00
Disponível para enviar em10 de abril de 2025Detalhes
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1 KG
R$ 990,00
About This Item
Código UNSPSC:
12352302
NACRES:
NA.85
R$ 990,00
Disponível para enviar em10 de abril de 2025Detalhes
Produtos recomendados
grau
Molecular Biology
for molecular biology
Nível de qualidade
esterilidade
non-sterile
Formulário
powder
composição
KH2PO4, 15 g/L
NaCl, 2.5 g/L
Na2HPO4, 33.9 g/L
NH4Cl, 5 g/L
aplicação(ões)
food and beverages
temperatura de armazenamento
room temp
adequação
nonselective for Escherichia coli
nonselective for coliforms
Descrição geral
M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed.
Aplicação
M9, Minimal Salts, 5X has been used:
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.
M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.
Características e benefícios
M9 powder provides:
- Standard formulation
- A budget-friendly alternative to liquid
Nota de preparo
1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.
2. Autoclave for 15 minutes at 121°C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 ml of M9 minimal salts, 5× concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.
Reconstituição
Make a 5x concentrated stock solution by stirring to suspend 56.4g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).
Dilute the 5x M9 concentrated stock to a 1x working solution by adding 200mL 5x M9 stock to 800mL sterile water. If needed, allow to cool before making additions, such as antibiotics (if desired).
produto relacionado
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Protocolos
General protocols for growth of competent cells in microbial medium.
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Where does the 5x concentrate need to be stored? At room temperature or at 4°C? And how long would the shelf life be?
1 answer-
Helpful?
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What is the preparation ratio for the culture medium?
1 answer-
The preparation instructions are listed on the product detail page in the DESCRIPTION section under 'Preparation'.
Please navigate to the link below:
https://www.sigmaaldrich.com/product/sigma/m60301. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121°C.
This convenient 5X concentrate can be stored and diluted as needed to prepare 5 L of 1X M9 minimal salts.For M9 minimal medium:
1. Aseptically dilute 200 mL of M9 minimal salts, 5X concentrate with 800 mL of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 mL of sterile 1 M glucose and 2 mL of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 mL of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.Helpful?
-
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