This product is soluble in water at a concentration of 10 mg/mL. It can also be dissolved in a buffer according to specific experimental references, such as 50 mM potassium phosphate. The stability of the solution has not been tested.
X2252
Xanthine Oxidase microbial
lyophilized powder, ≥7 units/mg solid
Sinônimo(s):
Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
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25 UNITS
R$ 1.050,00
100 UNITS
R$ 3.109,00
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25 UNITS
R$ 1.050,00
100 UNITS
R$ 3.109,00
About This Item
Produtos recomendados
fonte biológica
microbial
Nível de qualidade
Formulário
lyophilized powder
atividade específica
≥7 units/mg solid
peso molecular
~160 kDa
técnica(s)
inhibition assay: suitable
cor
brown
pH ideal
7.5-8.0
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)
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Descrição geral
Research area: Cell Cycle
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.[1]
Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.[1]
Aplicação
Xanthine Oxidase microbial has been used:
Ações bioquímicas/fisiológicas
Also contains proteolytic activity.
Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism.[3][5] This enzyme is also implicated in caffeine metabolism.[6] XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid.[7] Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.[8]
propriedades físicas
Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
Definição da unidade
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
forma física
Lyophilized powder containing BSA and sodium glutamate as stabilizers
Armazenamento e estabilidade
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Inibidor
Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Giuseppe Faudone et al.
Journal of medicinal chemistry, 64(11), 7156-7178 (2021-05-22)
The purine alkaloid caffeine is the most widely consumed psychostimulant drug in the world and has multiple beneficial pharmacological activities, for example, in neurodegenerative diseases. However, despite being an extensively studied bioactive natural product, the mechanistic understanding of caffeine's pharmacological
P J Marro et al.
Pediatric research, 41(4 Pt 1), 513-520 (1997-04-01)
The effect of allopurinol to inhibit purine metabolism via the xanthine oxidase pathway in neonates with severe, progressive hypoxemia during rescue and reperfusion with extracorporeal membrane oxygenation (ECMO) was examined. Twenty-five term infants meeting ECMO criteria were randomized in a
Pál Pacher et al.
Pharmacological reviews, 58(1), 87-114 (2006-03-02)
The prototypical xanthine oxidase (XO) inhibitor allopurinol, has been the cornerstone of the clinical management of gout and conditions associated with hyperuricemia for several decades. More recent data indicate that XO also plays an important role in various forms of
Michael C Hall
Molecular and cellular biochemistry, 292(1-2), 1-11 (2006-09-20)
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Protocolos
The appearance of uric acid is measured at 293nm by spectrophotometry.
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Good morning, I purchased xanthine oxidase X2252-25UN for quantifying SOD enzymatic activity. Which buffer should I use to solubilize the enzyme? After solubilization, how should I store the resulting solution?
1 answer-
Helpful?
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Guten Tag, in welchem Lösungsmittel und bis zu welcher Konzentration ist das Enzym gut löslich? Kann Natrium Phosphat Puffer verwendet werden? Danke.
1 answer-
For assay purposes, this product is prepared in 50 mM Potassium Phosphate Buffer, pH 7.5 at 25°C. The material is lyophilized from a solution containing BSA and sodium glutamate. While the solubility has not specifically been tested in sodium phosphate buffer, it is expected to be suitable. Please see the link below to review the assay protocol for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/938/x2252enz.pdfHelpful?
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